实体瘤间期细胞制备技术对荧光原位杂交效果的影响  

Influence of interphase nuclei preparation techniques on fluorescence in situ hybridlization results in solid tumors

作　　者：韩亚玲[1] 徐昕[1] 蔡岩[1] 王明荣[1] 

机构地区：[1]中国医学科学院中国协和医科大学肿瘤医院肿瘤研究所分子肿瘤学国家重点实验室,北京100021

出　　处：《中华医学遗传学杂志》2007年第3期331-333,共3页Chinese Journal of Medical Genetics

基　　金：国家重点基础研究发展规划（2004CB518705）;北京市科技计划重大专项（D0905001040331）;国家自然科学基金（30630067,30470969）

摘　　要：目的将改进的实体瘤间期细胞制备方法应用于荧光原位杂交,为实体瘤间期细胞的制备建立技术平台。方法采用随机引物法标记3号染色体着丝粒探针,分析实体瘤不同间期细胞制备技术对荧光原位杂交效果的影响。结果6种制片方法各具特点,根据其在应用方面有不同侧重,可采用不同制片方法。胶原酶法制片的荧光原位杂交效果最佳。对于冰冻组织或较小的组织,可采用印片法制片。结论适当的制片方法,结合荧光原位杂交,为实体瘤的染色体研究和诊断应用奠定了技术基础。Objective To establish a technology platform for the preparation of interphase nuclei for the fluorescence in situ hybridization （FISH） detection of solid tumor tissues. Methods The centromere probe of chromosome 3 was labeled by the random primer technique, and then hybridized to interphase nuclei prepared by six different methods in order to study the influence on FISH detection. Results Each method of slide preparation had its own characteristic, and could be used according to different needs. As regards to FISH, collagenase method got the best results. Whereas for frozen samples or small tissues, to prepare printing slides was more applicable. Conclusion The comparison of different slide preparation methods lays a technology foundation for the FISH application in cancer researches and clinical diagnosis of solid tumors.

关 键 词：间期核 荧光原位杂交 实体瘤 胶原酶 

分 类 号：R686[医药卫生—骨科学]