中国云南怒族群体线粒体DNA D环区SNP遗传多态性研究  

Study on SNP polymorphism of mitochondrial DNA D-loop region from Nu ethnic population in Ymman of China

作　　者：高树辉[1,2] 刘清波[1] 桂宏胜[1] 乔可[1] 李生斌[1] 

机构地区：[1]西安交通大学医学院法医学国家重点实验室,710061 [2]中国人民公安大学刑事科学技术系

出　　处：《中华医学遗传学杂志》2007年第3期354-357,共4页Chinese Journal of Medical Genetics

基　　金：公安部科研项目（20049121301）

摘　　要：目的观察怒族群体线粒体DNA单核苷酸多态性（single nucleotide polymorphism,SNP）的群体遗传特点,为法医学鉴定提供线粒体DNA序列多态性的基础数据,并为研究少数民族起源提供科学依据。方法应用ABI3730型遗传分析仪,PCR产物直接测序法,对87名中国云南怒族无关个体线粒体DNAD环Ⅰ区进行序列分析。结果在mtDNA高变区SNP16028～16362之间与Anderson序列比较,怒族87名无关个体中共检出62个碱基突变SNP位点,突变点492个,59个SNP单倍型。怒族mtDNA两个高变区SNP基因差异度为0.9675,偶合概率为0.0437。结论怒族线粒体DNAD环Ⅰ区SNP具有高度多态性,是对怒族群体的个体识别、亲权鉴定以及民族起源的分子研究有用的遗传标记。Objective To investigate the mitochondrial single nucleotide polymorphism （SNP） of Chinese Nu ethnic population from Yunnan region of China and to provide basic database for ethnic origin investigation and forensic purpose. Methods Genomic DNA from the whole blood of 87 unrelated individuals was extracted by standard chelex- 100, The sequence polymorphism was analyzed by PCR-based assay and using ABI 3730 Analyzer to detect many number of relatively common point mutations. Results Sixty-two SNP loci were observed among them with 492 point mutations and 59 haploids identified in mitochondrial DNA hypervariable region Ⅰ （ mtDNA HVS Ⅰ ）. The gene diversity was estimated to be 0.9675,and the random match probability was calculated to be 0,0437. Conclusion The result suggests that mtDNA HVS Ⅰ SNP database of Nu ethnic population can be a useful tool for forensic identity and original research.

关 键 词：怒族群体 线粒体DNA 单核苷酸多态性 个体识别 

分 类 号：R686[医药卫生—骨科学]