薄层扫描法测定解毒泄浊I号颗粒中大黄素的含量  

Determination of Emodin in Jieduxiezhu Ⅰ Granules by TLC-scanning

作　　者：张明生[1] 程志清[1] 

机构地区：[1]安徽中医学院第一附属医院药学部,安徽合肥230031

出　　处：《安徽中医学院学报》2007年第3期50-51,共2页Journal of Anhui Traditional Chinese Medical College

摘　　要：目的：建立解毒泄浊号Ⅰ颗粒中大黄素的含量测定方法。方法：薄层扫描法，展开剂：正己烷-乙酸乙酯-甲酸（30：10：0．5），检测波长440nm。结果：大黄素在0．2～1．0μg范围内呈良好线性关系，r=0．99859，平均回收率为98．22％，RSD为1．29％。结论：薄层扫描法简单、准确、重现性好，可用于解毒泄浊Ⅰ号颗粒中大黄素的含量测定。Objective： To establish a method for determination of emodin in Jieduxiezhu Ⅰ granules. Methods： Emodin in JieduxiezhuⅠ granules was determinated by TLC-scanning with a mixture of n-hexane-ethylacetate-formic acid （30： 10：0.5 ） as the developing system. The detection wavelength was 440 nm. Results ： The calibraction curve was linear in the range of 0.2 - 1.0 μg, r =0. 998 59 ,average recovery rate was 98.22% ,RSD = 1.29%. Conclusion ： The method was simple, accurate, good repeatability and suitable for the content determination of emodin in Jieduxiezhu Ⅰ granules.

关 键 词：解毒泄浊Ⅰ号颗粒 大黄素 薄层扫描法 含量测定 

分 类 号：R927.2[医药卫生—药学]