辛夷对豚鼠变应性鼻炎模型鼻黏膜P物质的影响  

Effects of flos magnoliae on substance P in nasal mucosa of guinea pigs with allergic rhinitis.

作　　者：吉晓滨[1] 谢景华[1] 杜洪[2] 藏林泉[3] 李雯[2] 谢军[3] 王磊[1] 

机构地区：[1]广州医学院附属市一人民医院耳鼻咽喉科,广州510180 [2]广州医学院附属市一人民医院病理科,广州510180 [3]中山大学基础学院药理教研室,广州510089

出　　处：《广东医学》2007年第6期860-863,共4页Guangdong Medical Journal

基　　金：广州市卫生局中医药中西医结合基金资助项目(编号:2004A011)

摘　　要：目的探讨辛夷对变应性鼻炎鼻黏膜P物质的影响。方法8只为正常豚鼠不做模型,用单纯橄榄油滴鼻作对照(对照组1)。用橄榄油将甲苯-2,4-二异氰酸酯配成浓度为10%溶液作为致敏剂,滴鼻,建立豚鼠变应性鼻炎动物模型16只,其中8只以后用生理盐水滴鼻做对照(对照组2),8只用0.5%生理盐水辛夷挥发油滴鼻(辛夷治疗组)。致敏结束、模型成功后,取3组鼻黏膜进行免疫组化染色,观察P物质在鼻黏膜中的分布情况。结果①对照组1:P物质分布于正常鼻黏膜上皮细胞、固有层和黏膜下层的血管内皮细胞、腺细胞及导管。对照组2:鼻黏膜上述部位P物质染色加深,密度增大。辛夷治疗组:鼻黏膜上述部位P物质染色变浅、密度减低。②3组P物质阳性细胞表达计数的比较表明:对照组1P物质阳性细胞计数低于对照组2(P<0.05),显示变应性鼻炎状态下鼻黏膜P物质阳性细胞计数增多,密度增大。辛夷治疗组鼻黏膜P物质阳性细胞计数低于对照组2(P<0.05),表明辛夷治疗变应性鼻炎后可以抑制鼻黏膜P物质的生成。辛夷治疗组的P物质阳性细胞计数与对照组1相当(P>0.05),表明辛夷治疗后,P物质阳性细胞计数可降到正常水平。结论辛夷可抑制变应性鼻炎鼻黏膜中P物质,用于治疗变应性鼻炎。Objective To investigate the effects of flos magnoliae on substance P in the nasal mucosa of guinea pig models with allergic rhinitis. Methods Eight animals treated with olive oil were used as blank controls （control group 1 ）. The other 16 guinea pigs with allergic rhinitis were treated with 10% Toluene 2,4 - diisocyanate. Among 16 animals in the theatment group, 8 were treated with saline（ control group 2）, the other 8 animals were treated with flos magnoliae （Flos magnoliae treating group）. After model was successfully established, the nasal mucosa were obtained from the 3 groups. Distribution and changes of substance P in nasal mucosa were observed by Immunohistochemical staining. Results Results showed that ① Control group 1 ： Substance P was present in the nomal nasal mucosal epithelial cells, epithelium cells of blood vessels, glandular cells and its ducts. Control group 2 ： The substance P staining density in the same nasal mucosa area increased. Immunocytes such as eosinophils, neutrophils, mast cells, plasmocytes and other cells were also substance P - positive. Flos magnoliae - treated group： The substance P staining density decreased. ② The number of substance P - positive, cells of control group 1 were significantly less than that of the control group 2 ,which showed that the substance P - positive cells of nasal mucosa increased on allergic nasal inflammation. The number of substance P - positive cells of flos magnoliae - treated group was significantly less than that of the control group 2, which showed that after treatment with flos magnoliae, the number of substance P - positive cells significantly decreased. The number of substance P - positive cells of flos magnoliae - treated group was similar to that of the control group 1 ,which showed that after treatment with flos magnoliae,the number of substance P - positive, cells can resume normal. Conclusion Flos magnoliae can inhibit substance P in the nasal mucosa with allergic rhinitis, and can treat allergic rhinitis.

关 键 词：辛夷 鼻炎 变应性 P物质 疾病模型 动物 免疫组织化学 

分 类 号：R713.6[医药卫生—妇产科学]