番茄P56和部分来源多糖裂解酶家族Ⅰ的其它蛋白的系统演化分析  

Phylogenetic Analysis of Tomato P56 and Some Other Proteins from Polysaccharide Lyase FamliyⅠ

作　　者：赵庆新[1] 张宇玲[2] 

机构地区：[1]江苏省滩涂生物资源与环境保护重点建设实验室盐城师范学院生命科学与技术学院,江苏盐城224002 [2]江苏省生物多样性和生物技术重点实验室南京师范大学生命科学学院微生物技术实验室,南京210097

出　　处：《武汉植物学研究》2007年第3期282-288,共7页Journal of Wuhan Botanical Research

基　　金：国家自然科学基金资助项目(30170005);江苏省教育厅高校自然科学指导性项目(01KJD18005);盐城师范学院博士科研启动基金

摘　　要：果胶裂解酶包含果胶酸裂解酶(pectate lyase)和果胶酸酯裂解酶(pectin lyase)二种形式,是一类多糖裂解酶,由细菌、真菌、植物和线虫等生物产生,分布在5个多糖裂解酶家族,果胶酶在食品与饮料、纺织与洗涤、制药等工业中有广泛的应用。利用NCBI BLASTX服务器,搜索与番茄P56蛋白氨基酸序列相似且都属于多糖裂解酶家族Ⅰ的果胶裂解酶蛋白序列共28条,用DNAMAN软件分析其保守区,用CLUSTALX8.0软件进行序列比对和Bi-oEdit软件进行文件转换,进一步用PUAP4.0软件构建系统进化树。构建的MP树(phylogenetic trees)和NJ树(neighbor-joining)显示:来自植物的果胶酸裂解酶、真菌的果胶酸酯裂解酶、细菌的果胶酸裂解酶分别可聚为一个独立的类群;相对于细菌果胶酸裂解酶和真菌果胶酸酯裂解酶而言,构巢曲霉(Aspergillus nidulans)的果胶酸裂解酶A和植物的果胶酸裂解酶之间有较近的亲缘关系;细菌Pseudomonas syringae的果胶酸酯裂解酶和真菌的果胶酸酯裂解酶之间的亲缘关系较近。Pectate and pectin lyases are a class of polysaccharide lyases produced by a diverse group of organisms including bacteria, fungi, plant and nematodes. Various pectate lyases and pectin lyases are divided into five families. By NCBI BLASTX server, tomato P56 amino-acid sequence was compared with other pectate and pectin lyases belonging to polysaccharide lyase family 1 28 proteins,with higher identity of amino acid sequence compared to P56 amino-acid sequence, were obtained. On the base of the constructing phylogenetic tree using PAUP 4. 0, MP （ phylogenetic trees） and NJ （neighbor-joining） trees of diverse pectate and pectin lyases showed that pectate lyases from plant, pectin lyases from fungi and pectate lyases from bateria belong to different groups, respectively. The pectate lyase A of Aspergillus nidulans is more closely related to plant pectate lyases than to pectin lyase from fungi and pectate lyases from bacteria. The pectin lyase from Pseudomonas syringae is more closely related to fungi pectin lyases than other lyases.

关 键 词：果胶酸裂解酶 果胶酸酯裂解酶 多糖裂解酶家族Ⅰ 系统演化树 

分 类 号：Q55[生物学—生物化学]