提高羊水细胞培养成功率的方法  

The improced of human amniotic fluid cells culture

作　　者：孙明强[1] 徐继秀[1] 孙立宁[1] 刘爱玲[1] 

机构地区：[1]潍坊市妇幼保健院,山东261011

出　　处：《中国优生与遗传杂志》2007年第6期45-46,共2页Chinese Journal of Birth Health & Heredity

摘　　要：目的提高羊水培养的成功率。方法正常羊水保留换液标本,以备复查;异常羊水采用细胞分离技术提取羊水中的有效细胞成分;及时发现污染羊水并进行抗菌处理。结果提高了羊水培养的成功率(99.4%)并缩短异常羊水的培养时间,解决了部分污染羊水的污染问题(2/3)。结论保留换液标本可有效解决因收集不当而导致无法诊断的问题;细胞分离技术可提高异常羊水细胞培养的成功率并缩短培养时间,污染的羊水也可抗菌后培养成功。Objective： To improve the successful rate of culture of human amniotic fluid cells. Methods ： To reserve the sample cell when exchange culture medium for reculture if there were mistakes while collecting the cultured amniotic fluid cells ; Separate the amniotic fluid cells from the abnormall amniotic fluid with isolated technique; Take remedial action with antibiotics in time when the culture bottle was infected. Results： The successful rate was improved （99. 4% ） , the culture time was shortened and the infected culture bottle were partly resolved （2/3）. Conclusion ： Reserving the sample ceils, refining amniotic fluid cells and antimicrobial if needed all can improve the successful rate of culture of human amniotic fluid cells.

关 键 词：羊水细胞培养 培养成功率 细胞分离技术 污染羊水 抗菌处理 培养时间 细胞成分 污染问题 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]