微孔板蛋白质芯片技术应用于单克隆抗体分型研究  

Micro-well Protein Array Technology Applied in Monoclonal Antibody Isotyping

作　　者：宋凯[1] 周佳菁[1] 叶赛[1] 彭海林[1] 王升年 肖华胜[1] 赵国屏[1] 张庆华[1] 

机构地区：[1]生物芯片上海国家工程研究中心/上海生物芯片有限公司,上海201203 [2]上海华冠生物芯片有限公司,上海201203

出　　处：《中国生物工程杂志》2007年第6期56-60,共5页China Biotechnology

基　　金：上海市优秀学科带头人计划(B类)(05XDB1414);国家"863"计划(2006AA020704)资助项目

摘　　要：设计与构建了可用于单克隆抗体分型鉴定的微孔板蛋白质芯片,利用该芯片进行了12株单克隆抗体和2种多克隆抗体的分型鉴定,并与ELISA方法进行了对比。结果表明,蛋白质芯片方法对单克隆抗体和多克隆抗体进行鉴定的结果,与ELISA方法进行鉴定的结果一致;与ELISA方法相比,蛋白质芯片的方法降低了试剂与样品的用量,缩短了工作时间,提高了工作效率。对于高通量的单克隆抗体制备体系,单克隆抗体分型蛋白质芯片是一种敏感、快捷的分型鉴定工具。Monoclonal antibody isotyping micro-well protein array was designed and established. Twelve monoclonal antibodies and two polyclonal antibodies were isotyped by the isotyping protein array and ELISA respectively. The results showed that there was no difference between protein array method and ELISA in the application of isotyping monoclonal and polyclonal antibodies. And fewer sample and materials were used and the efficiency was improved in protein array method compared with ELISA. As a result, the antibody isotyping protein array could be used as a sensitive, rapid, directional and economical tool applying in high throughput monoclonal antibody generation.

关 键 词：蛋白质芯片 单克隆抗体 分型 

分 类 号：Q51[生物学—生物化学]