Cartilage Regeneration by Delayed Engraftment of an Injectable Fibrin Clue/Hyaluronate Hybrid Scaffold Mixed with Mesenchymal Stem Cells  

作　　者：YANG Rui-fu HU Yun-yu WU Yin-song ZHANG Yu-fei WEI Yi-yong 

机构地区：[1]Department of Orthopaedics Xijing Hospital, the Fourth Military Medical University, Xi＇an 710032 [2]Department of Respiration, Xijing Hospital, the Fourth Military Medical University, Xi＇an 710032

出　　处：《Journal of US-China Medical Science》2007年第3期6-12,共7页美中医学（英文版）

摘　　要：Objective To evaluate the effect of an injectable hybrid fibrin/hyaluronate scaffold in cartilage defect by delayed engraftment. Methods A cartilage defect model was established by drilling deep into the femoropatellar grooves of rabbits with a diameter of about 4.5 millimeter. The defects were not treated until 42 days after drilling. An injectable scaffold was developed by combining fibrin glue and hyaluronate to mimic the structure of cartilage matrix. Autologous mesenchymal stem cells （MSCs） were induced to chondrocytes in vitro, and mixed with the injectable scaffold and then implanted into the cartilage defects. 30 rabbits （both sides of knees, n=60） were used in this study and they were randomly divided into three groups： group A （n=20） were treated by implanting the injectable scaffolds mixed with MSCs; group B （n=20） were treated by drilling the bottom of defect to reach medullary cavity; group C （n=20） were blank control. The regenerated cartilages were evaluated by both macroscopic observation and histological scores at 12 and 24 weeks after treatment. Results In group A, most cartilage defects were well repaired; the histological scores were significantly higher compared with group B and C （p〈0.01）. The reconstitution of the cartilage surfaces was also better in group A （p〈0.001）. There were no significant difference between 12 and 24 weeks in group A （p=0.917）. Conclusion this study revealed that by using autologous mesenchymal stem cells and an injectable fibrin clue/hyaluronate hybrid scaffold, defects of articular cartilage can be well repaired in a delayed manner, which has considerable relevance to the clinical practice.

关 键 词：cartilage defect tissue engineering cartilage regeneration 

分 类 号：R681.3[医药卫生—骨科学] R318[医药卫生—外科学]