不同产地不同品种柚皮中总黄酮和柚皮苷的含量比较  

Determination of total flavonoids and naringin in pummelo pericarp of various species from different habitats

作　　者：庞瑞[1] 杨中林[1] 

机构地区：[1]中国药科大学中药学院,南京210038

出　　处：《药学与临床研究》2007年第3期205-207,共3页Pharmaceutical and Clinical Research

摘　　要：目的:测定不同产地柚皮中总黄酮和柚皮苷的含量,筛选各种不同柚皮作为柚皮苷提取原料的可行性。方法:以紫外分光光度法测定不同产地柚皮中总黄酮的含量,检测波长为283 nm;用高效液相色谱法测定不同产地柚皮中柚皮苷的含量。流动相为甲醇-水-乙酸(v∶v∶v=35∶61∶4),柱温30℃,流速为1.0 mL.m in-1,检测波长为283 nm。结果:化橘红中总黄酮和柚皮苷的含量最高,胡柚皮次之,普通柚皮中总黄酮和柚皮苷含量都较少。结论:化州橘红是最理想的提取柚皮苷的原料,胡柚皮也可做为提取柚皮苷的药材资源。Objective：To determine the contents of total flavonoids and naringin in pummelo pericarp from vari-ous species and producing areas of China, and to study the feasibility of extracting naringin from pummelo peri-carp. Methods： The contents of total flavonoids were measured at 570 nm on a UV spectrophotometer. The con-tents of naringin were measured by the method of HPLC. Methanol extracts of samples and naringin reference substence were separated on a AichromBond-1 C18 column （4.6 mm x 250 mm,5μm） using a mixture solution of CH3OH-H20-HOAC （v： v： v = 35：61：4） as mobile phase and detected by UV detector at a wavelength of 283 nm. Results： The contents of total flavonoids and naringin in Exocarpium Citri Grandis are the highest, fol-lowed by the ripe fruit like Citrus paradisi cv. Changshanhuyou. , and pummelo pericarp at least. Conclusions ： With this method, extracting naringin from Exocarpium Citri Grandis is optimal, and pummelo pericarp and Cit-rus Paradisi cv. Changshanhuyou can also be used.

关 键 词：柚皮 化橘红 胡柚 总黄酮 柚皮苷 

分 类 号：R927.2[医药卫生—药学]