HPLC法测定头孢特仑新戊酯片的含量  

HPLC determination of content of cefteram pivoxil tablets

作　　者：陈云[1] 李小敏[1] 李薇[1] 刘春晖[1] 许向阳[1] 

机构地区：[1]江苏先声药物研究有限公司,南京210042

出　　处：《药学与临床研究》2007年第3期210-212,共3页Pharmaceutical and Clinical Research

摘　　要：目的：建立头孢特仑新戊酯片含量测定的高效液相色谱方法。方法：采用岛津CLASS VP C18（250mm×4．6mm，5μm）色谱柱；以乙腈：醋酸钠缓冲液（35：65）为流动相；以对羟基苯甲酸甲酯为内标；检测波长254nm；调整流量使头孢特仑新戊酯峰的保留时间为14min。结果：头孢特仑新戊酯的线性范围为100～1000μg·mL^-1，r=0.9999，平均回收率为99.63%，RSD为0.57%。结论：本法可用于头孢特仑新戊酯片的含量测定，操作简便，结果准确。Objective：To establish a HPLC method for the determination of cefteram pivoxil tablets. Method： Shimadzu CLASS VP C18 column （250 mm × 4.6 mm, 5μm） was selected, with a mobile phase consists of ace-tonitrile -0.05 mol·L^-1 sodium acetate buffer solution （35： 65 ）, at a detection wavelength as 254 nm. The in-ternal standard was methyl p-hydroxybenzoate, and the flow rate was adjusted to make the retention time of cefteram pivoxil about 14 minute. Results： The calibration curve was proved to be linear in the range of 100～1000μg·mL^-1（r=0. 9999）. The average recovery was 99.63% （RSD=0.57% ）. Conclusion： The proposed method is simple, sensitive and accurate.

关 键 词：HPLC 头孢特仑酯新戊酯片 含量 

分 类 号：R927.2[医药卫生—药学]