胰岛素对3T3-L1脂肪细胞中极低密度脂蛋白受体基因表达的影响  

Effect of Insulin on VLDLR Gene Expression in 3T3-L1 Adipocyte

作　　者：孙婷婷[1] 张木勋[1] 张建华[1] 袁刚[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院内分泌科,武汉430030

出　　处：《中国生物化学与分子生物学报》2007年第6期457-462,共6页Chinese Journal of Biochemistry and Molecular Biology

基　　金：国家自然科学基金(编号30400220);湖北省科技计划项目(编号2004AA301C35)~~

摘　　要：体外培养3T3-L1细胞分化模型,研究不同浓度胰岛素及慢性胰岛素刺激对3T3-L1脂肪细胞中极低密度脂蛋白受体(VLDLR)基因表达的影响.在不同浓度胰岛素及胰岛素慢性刺激的干预下,用半定量RT-PCR检测细胞VLDLRmRNA水平的变化.微量化GOD-PAP法检测培养基中残存的葡萄糖.在细胞诱导分化过程中,胰岛素浓度的增高促进VLDLR的表达;胰岛素慢性刺激下,VLDLR表达因浓度差异呈现不同变化.研究结果表明,胰岛素的浓度及慢性刺激对3T3-L1脂肪细胞的成熟和VLDLR基因的表达有显著作用,而胰岛素抵抗明显减低成熟脂肪细胞VLDLR的表达.The effect of different insulin concentration and chronic insulin stimulation on the very low density lipoprotein receptor （VLDLR） gene expression were researched in the differentiations of 3T3-L1 preadipocytes and in mature adipocytes. 3T3-L1 cells were induced to differentiate by using IBMX, dexamethasone and different concentration of insulin. Fully differentiated adipocytes were chronically stimulated by various concentration of insulin. And the relatively quantitative RT-PCR was used to detect the expression of VLDLR during 3T3-LI ceils differentiation and mature adipocytes. The microscale way of GOD-PAP was used to detect glucose remained in medium. Compared with control, the increasing insulin concentration boosted the gene expression of VLDLR （ P 〈 0.05）. VLDLR gene expression was dramatically changed under chronic insuhn stimulation in adipocytes, it depended on the concentration of insulin. Insulin plays an important role in induction of 3T3-L1 ceils, and it has great effect on VLDLR gene expression. Insulin resistance caused by chronic high insulin stimulation can down-regulated VLDLR gene expression in mature adipocytes （ P 〈 0.001）. These provide evidence to further establish a mechanism through which insulin regulate the VLDLR gene expression in adipocytes. Key words

关 键 词：脂肪细胞 胰岛素 极低密度脂蛋白受体 基因表达调控 

分 类 号：Q786[生物学—分子生物学]