乌司他丁对肠源性脓毒症大鼠D-二聚体和蛋白C系统的影响  

Effects of ulinastatin on serum D-dimer and protein C system in rats with severe gut-origin sepsis

作　　者：韩琪[1] 屠伟峰[1] 解立俊[1,2] 郄文斌[3] 

机构地区：[1]广州军区广州总医院全军临床麻醉中心,徐州医学院-广州军区广州总医院麻醉学硕士研究生培养基地,贵阳医学院在读硕士研究生510010 [2]贵阳医学院 [3]不详

出　　处：《实用医学杂志》2007年第11期1611-1613,共3页The Journal of Practical Medicine

基　　金：江苏省高校省级重点实验室开放课题项目(编号:KJS04001)

摘　　要：目的:观察乌司他丁(ulinastatin,UTI)对肠源性脓毒症大鼠D-二聚体(DD)、蛋白C(PS)系统的影响。方法:将Wistar大鼠随机分为4组,假手术组(SH组,n=36)、脓毒症组(SEP组,n=30)、UTI 3h给药组(UTI3h组,n=30)和12h给药组(UTI12h组,n=18)。利用经典盲肠结扎穿孔法建立肠源性脓毒症大鼠模型,分别于正常对照组及制模后6、12、24、48及72h组心脏取血测定DD、PC和蛋白S(PS)。结果:与SH组相比,SEP组大鼠DD在造模后逐渐升高,PC、PS含量降低,差异有显著性(P<0.05)。与SEP组相比,UTI3h、UTI12h组大鼠的DD降低,PC含量下降趋势减弱,差异有显著性(P<0.05)。结论:UTI能有效改善脓毒症期间的抗凝抑制和继发性纤溶亢进。Objective To observe the effects of ulinastatin （UTI） on serum D-dimer （D-D） and protein C （PC） system in rats with severe gut-origin sepsis. Methods One hundred and fourteen Wistar rats were randomly divided into four groups： sham group （n=36）, sepsis group （n=30）, UTI3h group （n=30）, and UTI12h group （n=18）. The rat model of gutorigin sepsis was established by classic cecal ligation and puncture （CLP）. Serum levels of D-D, PC, and protein S（PS） were detected in the normal and experimental rats 6, 12, 24, 48, and 72 h after CLP. Results The D-D level was higher but the levels of PC and PS were significantly lower in the sepsis group than in the sham group （P 〈 0.05）. As compared with those in the sepsis group, D-D was decreased and PC was increased markedly in the UTI3h or UTI12h group （P 〈 0.05）. Conclusion Ulinastatin can effectively improve the suppression of anticoagulation and resultant excessive fibrinolysis.

关 键 词：脓毒症 乌司他丁 纤溶 蛋白C系统 

分 类 号：R459.7[医药卫生—急诊医学]