电磁辐射对PC12细胞GluR2蛋白质表达及磷酸化的影响  

Effects of Electromagnetic Irradiation on the Level of GluR2 Protein Expression and Phosphorylation in PC12 Cells

作　　者：刘勇[1] 王登高[1] 张广斌[1] 余建华[1] 王虚步[1] 

机构地区：[1]第三军医大学军事预防医学院,重庆400038

出　　处：《中国辐射卫生》2007年第2期135-136,共2页Chinese Journal of Radiological Health

基　　金：国家自然科学基金项目(批号:30170237)

摘　　要：目的探讨电磁辐射对PC12细胞GluR2蛋白质表达及蛋白质磷酸化水平的影响。方法对离体培养的PC12细胞给予平均功率密度为90mW/cm2的电磁波持续辐照20min,采用westernblot方法检测辐照后0h、3h、12h、24h四个时相点GluR2蛋白质表达及蛋白质磷酸化水平的变化。结果电磁波辐照后0h和3hGluR2的蛋白质表达水平降低(P<0.05),其余各时相点无显著变化;辐照后0hGluR2的蛋白质磷酸化水平显著降低(P<0.01),3h后逐渐恢复至正常水平。结论平均功率密度为90mW/cm2的电磁波持续辐照20min能够降低PC12细胞GluR2的蛋白质表达和蛋白质磷酸化水平。Objective To observe the effects of electromagnetic irradiation on the level of GluR2 protein and phosphorrylation in PC12 cells. Methods The cuhured PC12 cells were exposed to 90 mW/cm^2 （ average power density） electromagnetic wave for 20 rain, The level of GluR2 protein and phosphorylation of PC12 cells were examined by western blot at 0 h , 3 h , 12 h , 24 h after irradiation. Results The level of GluR2 protein of PC12 cells decreased at 0 h and 3 h after irradiation; The level of GluR2 phosphorylation of PC12 cells decreased significantly at 0 h after irradiation. Conclusion 90 mW/ cm^2（ average power density）electromagnetic wave could lower the level of GluR2 protein and phosphorylation in PC12 cells.

关 键 词：电磁辐射 GLUR2 蛋白质表达 蛋白质磷酸化 PC12细胞 

分 类 号：R818.7[医药卫生—放射医学]