地锦草总黄酮最佳提取分离工艺的研究  

Study on optimal extraction and separation for total flavonoid of Euphorbia humifusa willd

作　　者：王淑萍[1] 乌垠[1] 孟祥颖[2] 张伟娜[1] 鲍永利[2] 李玉新[1] 

机构地区：[1]东北师范大学遗传与细胞研究所,吉林长春130024 [2]国家教育部农业与医药基因工程研究中心,吉林长春130024

出　　处：《分子科学学报》2007年第3期181-184,共4页Journal of Molecular Science

基　　金：国家自然科学基金资助项目(30472169);国家中医药管理局资助项目(04-05ZP21);吉林省发展与改革委员会资助项目([2005]1564);吉林省科技厅重大和技术攻关资助项目(20020502;20030905)

摘　　要：采用四因素三水平的正交实验方法,对地锦草总黄酮的最佳提取工艺进行了探索.采用乙酸乙酯萃取、酸碱沉淀、铅盐沉淀3种方法,对其最佳分离工艺进行了探索.正交实验结果表明:温浸法中四因素对地锦草黄酮的影响顺序为乙醇浓度>样液比>提取温度>提取时间.通过实验得到了地锦草总黄酮的最佳提取工艺:以10倍体积的50%的乙醇水溶液在80℃浸提1 h.地锦草总黄酮的最佳分离方法为酸碱沉淀法.这些数据为中药地锦草的开发和应用提供了科学依据.The orthogonal experiment was designed by using four factors and three levels, with water bath to study the optimal extractive techonology for total flavonoid of Euphorbia humifusa willd. It is found that the sequence in four factors of the warm immersed way is as following., the concentration of ethanol〉 the tatio of liquid and solid 〉extract temperature〉extract time. The optimal extractive condition is using 50% ethanol solution to E. willd solution extracted lh at 80℃ in the water bath. The optimal isolative technology is acid-base precipitation. All of these information provide scientific material and basis for large-sale development and application of the Chinese traditional and herbal medicine.

关 键 词：地锦草 黄酮 正交实验 提取工艺 

分 类 号：Q81[生物学—生物工程]