单螺旋和3股螺旋蚕丝纤维支架的力学性能及其细胞黏附性能比较  

Mechanical performances and cell adhesion functions of simple spiral silk scaffold versus 3-strand spiral silk scaffold

作　　者：王洪[1] 李艳军[1] 杨述华[1] 严力军[1] 吴宏斌[1] 邵增务[1] 段德宇[1] 孟春庆[1] 袁文旗[1] 赵玉鑫[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院骨科,湖北省武汉市430022

出　　处：《中国组织工程研究与临床康复》2007年第18期3523-3526,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：湖北省科技攻关计划(2006AA301C32)~~

摘　　要：目的:经蚕茧缫丝脱胶而成的丝状纤维是一种无生理活性的天然结构性蛋白,这种结构使丝状支架具有良好的力学性质和生物相容性。观察采用单螺旋和3股螺旋蚕丝两种不同编织方法制备的丝状支架力学性能和细胞黏附性能的差异。方法:实验于2005-10/2006-05在华中科技大学同济医学院附属协和医院骨科实验室完成。将蚕丝用不同的编织方法制备单螺旋、3股螺旋两种丝状支架,直径4.5mm。实验方法:①丝状支架的力学检测:两种丝状支架各取10cm置于AGS-H多功能材料力学实验机的夹具中,固定后以10mm/min的速度行拉断试验。②对SD大鼠骨髓基质干细胞分离培养。实验评估:①通过拉断实验测定最大载荷、最大拉伸长度及弹性模量。②对骨髓基质干细胞进行苏木精-伊红染色。③扫描电镜观测骨髓基质干细胞与丝状支架的黏附情况。结果:①力学性能检测结果:单螺旋、3股螺旋两种丝状支架的最大载荷、最大拉伸长度、弹性模量[分别为(870±32),(846±20)N;(14.60±0.85),(13.90±0.64)mm;(6519±287),(6373±375)MPa]差异无显著性意义(P>0.05)。②免疫组织化学鉴定结果:骨髓间充质干细胞的细胞膜抗原CD44有阳性表达,CD34均为阴性表达。③骨髓基质干细胞与丝状支架的黏附情况:两种编织方法的丝状支架的丝状纤维表面均光滑,有较多的骨髓间充质干细胞黏附在丝状纤维上。结论:单螺旋和3股螺旋两种丝状支架的力学性能和细胞黏附性能相近。AIM： Silk fiber made of degumming silkworm cocoon filature is a kind of natural structural albumen without physiological activity, which make silk scaffold have good mechanical function and biocompatibility. This study was aimed to observe the differences of mechanical and adhesion function between simple spiral silk scaffold and 3-strand spiral silk scaffolds. METHODS： The experiment was performed at the Laboratory of Department of Orthopaedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology from October 2005 to May 2006. The silk was made into simple spiral, 3-strand spiral scaffolds, with the diameter of 4.5 mm. Experimental methods： （1）Mechanical test of silk trestle： 10 cm of the two kinds of scaffolds were put in clamping apparatus of AGS-H multifunctional mechanical instrument. The pull and break test was done at a speed of 10 mm/min after fixation. （2）Bone marrow stromal cells （BMSCs） of SD rats were isolated and cultured. Experimental evaluation： （1）Maximum load, maximal draw-extension length and elastic modulus were tested by pull-break test. （2）BMSCs were recongnized by haematoxylin-eosin （HE） staining. （3）Adherence of BMSCs on silk scaffold was observed by scanning electron microscope. RESULTS： （1）Mechanical determination： Maximum load, maximal draw-extension length and elastic modulus in simple spiral, and 3-strand spiral scaffolds were [（870±32）, （846±20）N; （14.60±0.85）, （13.90±0.64）mm ; （6 519±287）, （6 373±375）MPa], and there was no significant difference （P 〉 0.05）. （2）Immunohistochemical identification： The expression of CD44 was positive in BMSCs, yet CD34 was negative. （3）Adhension of BMSCs on silk scaffold： The silk scaffold had smooth surface and many BMSCs adhered to the silk fibers. CONCLUSION： Mechanical performances and cell adhesion of simple spiral and 3-strand spiral scaffolds are similar.

关 键 词：蚕丝 骨髓基质干细胞 丝状支架 生物材料 

分 类 号：R318.08[医药卫生—生物医学工程]