明胶/白芨胶载药多孔材料的组织相容性评价(英文)  

Histocompatibility of a porous material:Gelatin-bletilla carrying traditional Chinese medicine

作　　者：彭锐[1] 邹阳[2] 程井军[1] 

机构地区：[1]湖北中医学院针灸骨伤系,湖北省武汉市430061 [2]湖北省中医药研究院,湖北省武汉市430074

出　　处：《中国组织工程研究与临床康复》2007年第18期3644-3646,共3页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：湖北省科技攻关项目(2004AA303B03)~~

摘　　要：背景:一些实验已证明,某些中药制剂在与人体组织、体液或血液接触和相互作用时,具有良好的生物相容性,因此可以与生物材料复合,使材料的理化性能以及功用得到改善。目的:评价明胶/白芨胶载药多孔材料的组织相容性。设计:单一样本实验。单位:湖北中医学院针灸骨伤系。材料:实验于2005-02/04在华中科技大学同济医学院骨科完成。选择小白鼠12只,雌雄各半,体质量18～24g;日本大耳白兔6只,兔龄9～10个月,体质量2.8～3.0kg,饲养温度:(18±1)℃,单笼饲养。方法:冷冻干燥法制备海绵状明胶/白芨胶载药多孔材料,把中药黄连、丹参提取物复合到其中;急性全身毒性实验:12只小鼠随机分为实验组和对照组,实验组动物由腹腔注射标准浓度材料浸提液,剂量为50mL/kg;对照组注射与浸提液同批号的生理盐水,剂量为50mL/kg,注射后24,48,72h观察动物的一般状态,并记录注射后24,48,72h体质量变化。通过体内植入法,将该载药材料植入兔背部肌肉内,术后1,2,6周麻醉后各处死2只兔,取材。通过大体观察和组织学检查观察生物材料在体内的组织反应。主要观察指标:生物材料在兔体内组织反应的大体观察和组织学检查观察。结果:12只小鼠和6只大耳白兔全部进入结果分析。急性全身毒性实验:所有实验小鼠一般情况良好,活动、食欲正常,呼吸平稳,无腹部刺激症状、衰竭、发绀以及死亡现象。注射后24,48,72h小鼠体质量均呈增长趋势,实验组与对照组比较未见明显差异(P>0.05)。植入实验:①大体观察:1周时的取材标本与肌肉纤维组织结合紧密,4周时的取材标本与肌肉纤维组织已融合在一起,不易分离;8周时取材在兔原来的材料植入部位已经看不见明胶/白芨胶载药多孔材料的存在。②组织学观察:明胶/白芨胶载药多孔材料植入1周时,材料标本周围有较多的炎性细胞和间充质细胞,4周时材�BACKGROUND：Some experiments have proved that some traditional Chinese medicines （TCM） have good biocompatibility when they touch and interact with human body tissues, body fluid or blood. Therefore, the physicochemical properties and functions of biomaterials can be improved by compounding it with some TCM. OBJECTIVE： To evaluate the biocompatibility of gelatin-bletUla porous material carrying TCM DESIGN ： Single sample experiment SETTING： Department of Acupuncture ＆ Moxibustion and Orthopaedics, Hubei College of Traditional Chinese Medicine. MATERIALS： This experiment was carried out in the Department of Orthopaedics, Tongji Medical College, Huazhong University of Science and Technology between February and April 2005. Twelve mice, male and female in half, weighing 18-24 g , and 6 Japanese big-eared rabbits, weighing 2.8 to 3.0 kg, were chosen for this experiment. They were raised at（18＋1 ）℃ in separate cages. METHODS： Spongy gelatin-bletilla porous materials carrying extract of Coptidis and Radix Salviac Militiorrhizae was prepared by freeze-drying method. The acute systemic toxicity test： The 12 mice were divided into experimental group and control group. Mica in the two groups were intraperitoneally injected with leaching liquor and normal saline, respectively （the dose was 50 mL/Kg）, then the general state and body mass of mice were recorded at the 4^th, 24^th, 48^th and 72^rd hours after injection, respectively. The porous materials were implanted in dorsal muscle of rabbits. Two rabbits were sacrificed at the 1^st, 2^nd and 6^th weeks after operation seperately, and tissue reaction of biomaterial in vivo was assessed by gross observation and histological examination. MAIN OUTCOME MEASURES： Gross observation and histological examination of tissue reaction of biomaterials in vivo. RESULTS： Twelve mica and six big-eared rabbits were involved in final analysis. The acute systemic toxicity test： Mica of two groups had good status, normal activity and appetite, stable respir

关 键 词：生物材料 明胶 白芨 中药 组织相容性 

分 类 号：R318.08[医药卫生—生物医学工程]