四种缓解化学药物治疗后骨髓抑制中成药对环磷酰胺所致小鼠血虚模型外周血和骨髓象的影响  

Influences of four kinds of Chinese medicines that can relieve marrow restraint after chemotherapy on peripheral blood and bone marrow smear in model mice of hematopenia induced by cyclophosphamide

作　　者：苗明三[1] 周立华[1] 侯江红[1] 陈纲领[1] 张玉林[1] 

机构地区：[1]河南中医学院艾滋病研究所,河南省郑州市450000

出　　处：《中国组织工程研究与临床康复》2007年第20期3998-4000,共3页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：国家"十五"省部联动项目(2004BA719A13-06);国家"十五"科技攻关艾滋病专项(2004BA719A09-0204)~~

摘　　要：目的:观察补气养血、健脾益肾的参芪颗粒,补气养血的复方阿胶浆,补气养阴的贞芪扶正颗粒,温肾健髓、益气养阴、生血止血的复方皂矾丸4种中成药对环磷酰胺所致小鼠血虚模型外周血及骨髓象的影响。方法:实验于2005-06/09在河南中医学院药理实验室完成。①选用健康昆明种成年小鼠150只,雌雄不拘。按随机数字表法分为15组,每组10只:高、中、低剂量贞芪扶正颗粒组分别按15,10,5g/kg(20mL/kg)剂量灌胃贞芪扶正颗粒混悬液(主要成分:黄芪、女贞子;甘肃扶正药业科技股份有限公司生产,批号040803);高、中、低剂量复方阿胶浆组分别按10,20,30mL/kg剂量灌胃复方阿胶浆(主要成分:阿胶、熟地黄、党参等;山东东阿阿胶股份有限公司生产,批号050446);高、中、低剂量复方皂矾丸组分别按2.4,1.6,0.8g/kg(20mL/kg)剂量灌胃复方皂矾丸混悬液(主要成分:皂矾、西洋参、海马等;陕西郝其军制药有限责任公司生产,批号041014);高、中、低剂量参芪颗粒组按3,2,1g/kg(0.2mL/10g)剂量灌胃参芪颗粒混悬液(主要成分:人参、黄芪、当归等;江西山高制药有限公司生产,批号040702);鲨肝醇组按10g(20mL)/kg剂量灌胃鲨肝醇混悬液;空白组和模型组灌胃同体积的生理盐水(0.2mL/10g)。每天给药1次,连续给药10d。从实验的第6天开始除空白组外,各组小鼠腹腔注射环磷酰胺40mg/kg,连续给药5d。②各组小鼠分别于末次给药后24h,进行血常规检测。另取右侧股骨,镜下计数骨髓有核细胞数。③计量资料符合正态分布、方差齐者用t检验。结果:小鼠150只均进入结果分析。①对外周血的影响:模型组小鼠血白细胞、红细胞和血小板计数及血红蛋白水平、血细胞比容均明显低于空白组(t=2.49～8.19,P<0.05￣0.01)。鲨肝醇组、各剂量参芪颗粒组和复方阿胶浆组及贞芪扶正颗粒组、复方皂矾丸组小鼠血白细胞计数均明显高于模型�AIM： To observe the influences of Shenqi granule that can invigorate vital energy, nourish blood, invigorate the spleen and tonify kidney, compound E Jiao slurry that can invigorate vital energy and nourish blood, Zhenqi Fuzheng granule that can invigorate vital energy, nourish yin, and compound Zaofan pill that can warm kidney, strengthen marrow, supplement qi and nourish yin, have the effects of hematosis and hemostasia on peripheral blood and bone marrow smear in model mice of hematopenia induced by cyclophosphamide. METHODS： The experiment was accomplished at Pharmacology Laboratory of Henan University of Traditional Chinese Medicine from June to September 2005. （1）Totally 150 healthy Kunming adult mice of either sex were selected and randomly divided into 15 groups with 10 mice in each. Mice of high, moderate and low dose of Zhenqi Fuzheng granule groups were given 15,10,5 g/kg （20 mLIkg）Zhenqi Fuzheng granule suspension （main component： radix astragali, glossy privet fruit, produced by Gansu Fuzheng Pharmacy Co., Ltd. Lot number 040803） by gastric perfusion. Mice of high, moderate and low compound E Jiao slurry groups were given 10,20,30 mL/kg compound E Jiao slurry （main component： eguus asinus, prepared rehmannia root, radix codonopsitis, produced by Shandong Dong-E E-jiao Co., Ltd. Lot number 050446） by gastric perfusion. Mice of high, moderate and low compound Zaofan pill groups were given 2.4, 1.6,0.8 g/kg （20 mLIkg）Zaofan pill suspension （main component： melanteritum, panax quinquefolium L., seahorse, produced by Shaanxi Haoqijun Pharmacy Limited-liability Company, lot number 041014） by gastric perfusion. Mice of high, moderate and low Shenqigranule groups were given 3,2,1 g/kg （0.2 mL/10 g）Shenqigranule suspension （main component： ginseng, radix astragali, angelica, produced by Jiangxi Shangao Pharmacy Co., Ltd. Lot number 040702） by gastric perfusion. Mice of Shaganchun group were given 10 g （20 mL）/kg Shaganchun suspension by gastric perfusion. Mice o

关 键 词：中成药 血虚模型 血象 

分 类 号：R322.2[医药卫生—人体解剖和组织胚胎学]