二次萃取法测定人血浆中芬太尼浓度  

Determination of Concentration of Fentanyl in Human Plasma by HPLC With 2-step Extraction

作　　者：尤培军[1] 

机构地区：[1]山东省济宁市第一人民医院,山东济宁272011

出　　处：《中国现代医生》2007年第06Z期41-42,共2页China Modern Doctor

摘　　要：目的探讨高效液相色谱法-紫外二次萃取法测定人血浆中芬太尼浓度的方法。方法本实验采用内标法,以盐酸瑞芬太尼为内标,以Zorbax-CN(250mm×46mm,5μm)为固定相,以含0.015mol/L磷酸氢二钠和0.05mol/L戊烷磺酸钠的乙腈-水溶液(70:30,v/v)为流动相,流速1.5mL/min,紫外检测波长200nm。结果标准曲线在1～100ng/mL范围内线性关系良好(r=0.999),最低检测浓度为1ng/mL,方法回收率为(91.45±4.55)%,提取回收率为(95.63±4.02)%,日内变异RSD(6.23±2.90)%,日间变异RSD(6.45±4.12)%。结论本方法简便、准确、检测浓度低,能够满足血浆中低浓度芬太尼的测定及临床药代动力学研究的要求。Objective To determine the concentration of fentanyl, in human plasma by HPLC with ult raviolet detection. Methods fentanyl was separated on Zorbax-CN coLumn with a mobile phase of water （containing 0.015 moL/L Na2HPO4 and 0.05 mol/L Pentane Sulfonic Acid Sodium Salt）-acetonitrile （70：30） and detected at 200 nm. Internal standard was remifentaniL The detection wavelength was 200nm. Results Calibration curve for fentanyl was linear in the range of 1-100ng/mL （r=0.999）.The detection limit was 1.0ng/mL.The within-day RSD was （6.23±2.90） %, the between-day RSD was （6.45± 4.12）%.The rote of the method recovery was （91.45±4.55）%.The rate of the extraction recovery was （95.63±4.02）%. Conclusion This method was quick and accurate for the determination of fentanyl in human plasma and useful for pharmakinetic study of fentanyl.

关 键 词：高效液相色谱法 芬太尼 血浆 

分 类 号：R971[医药卫生—药品]