磁处理对滨梅组培苗增殖及再生的影响  

Effects of Magnetic Treatment of Beach Plum in vitro Subculture Explants on the Regeneration and Growth

作　　者：闫道良[1] 钦佩[1] 吴卫国[2] 王光[1] 宰学明[1] 孙东林[1] 

机构地区：[1]南京大学盐生植物实验室,江苏南京210093 [2]南京大学物理学系,江苏南京210093

出　　处：《南京林业大学学报（自然科学版）》2007年第3期99-102,共4页Journal of Nanjing Forestry University：Natural Sciences Edition

基　　金：国家林业局"948"创新项目(2005-4-43)

摘　　要：通过外加不同强度的电磁场处理滨梅茎段外植体,以促进其不定芽的增殖。结果表明:对滨梅外植体施加强度为97 kA/m磁场,处理10 min,可明显地促进不定芽的增殖,其增殖倍数是对照的2.3倍。将经过97 kA/m磁处理的外植体接种在培养基MS+ZT(2 mg/L)+IBA(0.2 mg/L)+Vc(30 mg/L)上,芽的增殖倍数与幼苗高度、鲜重、干重都优于接种在培养基MS+ZT(2 mg/L)+NAA(0.2 mg/L)+Vc(30 mg/L)和对照的情况,经97 kA/m磁处理的外植体接种于优化生根培养基1/2MS+NAA(0.1 mg/L)+IBA(0.1 mg/L)+牛血清白蛋白(30 mg/L),诱导的不定根平均根长(5.39±0.68)cm,显著高于对照。说明培养基的优化与一定强度的磁场能有效地促进滨梅组培苗的增殖分化与生长。The magnetic field is applied as a strength gradient （48～115 kA/m）. It is shown that the increase in the field strength stimulates the regeneration and growth in the explants. Effects of a ten-minute treatment of explants with a strength of 97 kA/m on the regeneration and growth are significant, and the number of the sprouts induced is 2.3 times that the number of control group. The fresh weight, dry weight and height of the sprouts, from the ex- plants were treated with a strength of 97 kA/m, in culture medium MS＋ZT（2 mg/L）＋IBA （0.2 mg/L）＋Vc（30 mg/L） are more significant than those in MS＋ ZT（2 mg/L）＋NAA （0.2 mg/L）＋Vc（30 mg/L） and control group. It is greatly significant at 0.01 level that the root length of plantlets from explants treated with a strength of 97 kA/m and put into optimized rooting medium 1/2MS＋NAA（0. 1 mg/L）＋IBA（0. 1 mg/L）＋bovine serum albumin 30 mg/L, compared with the control group. The average root length is （5.39±0. 68）cm. It is possible that the optimization of culture medium and treatment of explants with a certain magnetic field strength can increase the number of regeneration sprouts and can promote growth.

关 键 词：磁处理 滨梅 组培苗 增殖再生 

分 类 号：S722.37[农业科学—林木遗传育种]