肝力注射液逆转人肝癌细胞获得性多药耐药的体外研究  

In vitro study of Ganli Injection on reversing acquired multidrug resistance of human hepatocellular carcinoma

作　　者：顾伟[1] 翟笑枫[1] 张亚妮[1] 凌昌全[1] 

机构地区：[1]第二军医大学长海医院中医科,上海200433

出　　处：《中草药》2007年第6期871-874,共4页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(30600814);上海市科委基金资助项目(05ZR14144)

摘　　要：目的研究肝力注射液体外逆转人肝癌细胞5-Fu获得性多药耐药的作用。方法MTT法检测肝力注射液对人肝癌BEL-7402/5-Fu耐药细胞株的增殖抑制率及对5-Fu获得性多药耐药的逆转指数,倒置显微镜及Wright-Giemsa染色观察细胞形态学改变,流式细胞术检测细胞内阿霉素荧光强度。结果人肝癌BEL-7402/5-Fu细胞株对临床常用化疗药物显示出不同程度的交叉抗药性,肝力注射液(以苦参碱计)在0.230、0.115、0.058mg/mL3个质量浓度对人肝癌BEL-7402耐药细胞株72h的增殖抑制率分别为(9.25±2.38)%、(8.46±1.90)%、(4.23±2.05)%,对5-Fu的逆转指数分别为5.50、3.83、2.25,肝力注射液在0.230mg/mL质量浓度预处理细胞2h能提高细胞内阿霉素的量约38.61%。结论肝力注射液在体外具有逆转人肝癌细胞5-Fu获得性多药耐药的作用。Objective To study the reversal effect on acquired muhidrug resistance （MDR） of human hepatocellular caricinoma （HCC） by Ganli Injection. Methods Proliferative inhibition rate of HCC BEL- 7402/5-Fu cell line and reversal index of 5-Fu acquired MDR were measured by MTT assay. Cell morphological changes were observed under inverted microscope and Wright-Giemsa stain method. Fluorescence intensity of Adriamycin （ADM） was detected by Flow cytometry. Results HCC BEL-7402/ 5-Fu cell line exhibited cross-resistance to general chemotherapeutics in clinic. Proliferative inhibition rates of HCC BEL-7402/5-Fu cell line treated for 72 h by Ganli Injection at the （matrine） concentration of 0. 230, 0. 115, 0. 058 mg/mL were （9.25±2.38）%, （8.46±1.90）%, and （4.23±2.05）%, respectively. The corresponding reversal indexes to 5-Fu were 5.50, 3.83, and 2.25. Pre-treatment of Galli Injection at the concentration of 0. 230 mg/mL for 2 h could increase the accumulation of ADM in cells to 38. 61%. Conclusion Ganli Injection has the effect on reversing 5-Fu acquired MDR of human hepatocellular carcinoma in vitro.

关 键 词：肝力注射液 苦参碱 盐酸川芎嗪 肝癌(HCC) 多药耐药(MDR) 

分 类 号：R286.91[医药卫生—中药学]