染料木素诱导人胃癌BGC-823细胞凋亡及其分子机制的研究  

Apoptosis induced by genistein in human gastric cancer BGC-823 cells and its molecular mechanisms

作　　者：苏文[1] 刘艳平[1] 李忠魁[1] 张树冰[1] 郑杰[1] 吴丽萍[1] 李宇晟[2] 

机构地区：[1]中南大学生物科学与技术学院细胞生物学系,湖南长沙410013 [2]中南大学湘雅医学院,湖南长沙410013

出　　处：《中草药》2007年第6期874-878,共5页Chinese Traditional and Herbal Drugs

基　　金：湖南省科学技术厅基金资助项目(03ssy3091)

摘　　要：目的 探讨染料木素（genistein）体外抑制人胃癌BGC～823细胞生长及诱导其凋亡的作用机制。方法 采用四甲基偶氮唑盐（MTT）法检测染料木素对人胃癌BGC-823细胞的增殖抑制效应；DAPI荧光染色观察细胞凋亡形态；流式细胞仪检测染料木素作用24h后BGC-823细胞的周期分布和凋亡率；RT—PCR方法检测环氧合酶-2（COX-2）在mRNA水平的表达；进一步采用Westernblotting法检测COX-2在蛋白质水平的表达。结果 染料木素对BGC-823细胞具有增殖抑制效应，并有时间及浓度依赖性。染料木素作用24、48、72h的半数抑制浓度（IC50）分别为（81．04±1．03）、（31．85±2．26）、（20．73±2．11）μg／mL。10～80μg／mL染料木素作用24h后，BGC-823细胞出现核碎裂，呈现典型的凋亡特征，细胞被阻滞于G2／M期，随药物质量浓度的增加，细胞凋亡率逐渐增加，细胞内COX-2表达在mRNA水平和蛋白质水平均下调。结论 染料木素对人BGC-823细胞有较强的抑制增殖和诱导凋亡作用，其机制可能与抑制COX-2表达有关。Objective IO investigate the effect of genistein on COX-2 expression in human gastric cancer BGC-823 cells and to explore its potential mechanisms of inhibiting proliferation and inducing apoptosis. Methods MTT Assay was used to observe the effect of genistein after 24 h on proliferation of BGC-823 cells. Cell cycle and apoptosis were analyzed by flow cytometry （FCM） and DAPI staining. Transcription level of COX-2 was detected by RT-PCR and protein level of COX-2 was detected by Western blotting. Results Genistein significantly inhibited the proliferation of BGC-823 cells in dose- and time-dependent manners, the IC50 value of genistein at 24, 48, and 72 h were （81.04±1.03）, （31.85± 2.26）, and （20. 73±2.11） vg/mL, respectively. When treated with 10-80 μg/mL genistein for 24 h, BGC-823 cells were arrested at G2/M phase, and the apoptotic rate was increased along with the increase of genistein concentration, meanwhile, COX-2 expression at transcription and protein level was decreased. Conclusion Genistein has a stronger inhibition on proliferation and induction on apoptosis of BGC-823 cells through inhibiting COX-2 expression.

关 键 词：染料木素 BGC-823细胞 增殖 环氧合酶-2 凋亡 

分 类 号：R286.91[医药卫生—中药学]