AgNO_3在铁皮石斛组织培养中抗衰老作用  

Effects of AgNO_3 on caducity-resistant from tissue culture of Dedrobium officinale

作　　者：李进进[1] 廖俊杰[1] 许继勇 麦瑜玲 

机构地区：[1]广东轻工职业技术学院食品与生物工程系,广东广州510300 [2]汕头市中蔬花卉有限公司,广东汕头515041

出　　处：《中草药》2007年第6期914-917,共4页Chinese Traditional and Herbal Drugs

基　　金：广东省2005年科技资助项目[粤科计字(2005)193号];汕头市2005年科技资助项目[汕市财文(2005)219号]

摘　　要：目的解决铁皮石斛在组织培养过程中的衰老问题。方法在原球茎增殖培养基、丛芽增殖培养基和生根培养基中分别加入AgNO30、1、2mg/L,测定乙烯的产生量,观察试管苗生长情况。结果AgNO3抑制乙烯的产生,提高了原球茎的增殖速度和丛芽的分化效率,明显促进种苗的生长,提高移栽成活率2倍,表现出抗衰老功能。结论1mg/LAgNO3可以作为铁皮石斛组织培养的抗衰老剂。Objective To solve the caducity question of Dendrobium officinale during the tissue-culture process. Methods Added the different concentration of 0. 1 and 2 mg/L AgNO3 into the PLB proliferation medium, buds proliferation medium, and rooting medium separately. The quantity of the ethylene production was to be determined and the situation of the tube seedling growth was to be observed. Results AgNO3 can suppress the production of the ethylene, enhance the multiplication speed of PLB and split-up efficiency the clump of bud, and obviously promote the seedling＇s growth. It showed that the survival ratio of transplant could enhance as many as two times and display the merit of caducity-resistant. Conclusion AgNO3 （1 mg/L） can be taken as the caducity-resistant reagent of D. officinale during the tissue-culture processing.

关 键 词：铁皮石斛 组织培养 AGNO3 抗衰老 

分 类 号：R282.1[医药卫生—中药学]