γ射线诱导DNA损伤中DNA浓度和剂量率的影响  

Effect of Concentration of DNA and Dose Rate in DNA Damage Induced by γ Ray

作　　者：孔福全[1] 王潇[1] 倪嵋楠[1] 隋丽[1] 杨明建[1] 赵葵[1] 

机构地区：[1]中国原子能科学研究院,北京102413

出　　处：《原子核物理评论》2007年第2期103-107,共5页Nuclear Physics Review

基　　金：国家自然科学基金资助项目(10175095;10435020);中国原子能科学研究院院长基金资助项目;中国原子能科学研究院核物理研究所所长基金资助项目(12SZZ-200602)~~

摘　　要：在进行辐射致DNA损伤中,DNA浓度和剂量率是两个重要的因素。利用γ射线在不同的剂量率下对不同浓度的质粒DNApUC19水溶液进行了辐照,利用凝胶电泳对辐照后的样品进行分析。结果表明,在同一剂量下被辐照的DNA样品,随浓度的降低DNA损伤越来越严重;通过分析软件和理论公式得到的每个DNA上的双链断裂数随着DNA浓度的变化呈现为非线性趋势。As two factors, the changes of concentration of DNA and dose rate are of significance in the study of DNA damage induced by irradiation. The influence of DNA damage induced by γ ray at different concentration of DNA under different dose rate was investigated. The result of gel electrophoresis indicate that DNA molecules were damaged severely as the concentration of DNA decreasing. The number of double strand breakages per DNA were obtained by the software of Alpha Innotech and theoretical analysis. It shows that the numbers of double strand breakages per DNA presented nonlinear feature as the concentration of DNA decreasing, and the dose rate had little influence on DNA damage.

关 键 词：Γ射线 DNA损伤 DNA浓度 剂量率 pUC19 

分 类 号：Q691[生物学—生物物理学] TN16[电子电信—物理电子学]