辐射诱导促进AdCMV-p53转染前列腺癌细胞  

High-efficiency Transfer and Expression of AdCMV-p53 in Human Prostate Adenocarcinoma Cells Induced by Low-dose ^(60)Co γ-ray

作　　者：张红[1] 刘兵[1] 高清祥[2] 谢漪[1] 郝冀方[1] 段昕[1] 周清明[1] 王燕玲[1] 

机构地区：[1]中国科学院近代物理研究所,甘肃兰州730000 [2]兰州大学生命科学院,甘肃兰州730000

出　　处：《原子核物理评论》2007年第2期112-116,共5页Nuclear Physics Review

基　　金：国家自然科学基金资助项目(1075151);甘肃省科技攻关计划资助项目(2GS052-A43-008-02);甘肃省自然科学基金资助项目(3ZS061-A25-021)~~

摘　　要：用腺病毒重组体(AdCMV-p53/GFP)转染经0.5,1.0和2.0Gyγ射线辐射处理的前列腺癌细胞[PC-3(nullp53)],用克隆形成法检测细胞增殖能力,用流式细胞分析法测定腺病毒重组体转染率和外源性p53蛋白表达。结果提示,辐射诱导使腺病毒重组体转染PC-3细胞提高7%—39%。辐射联合AdCMV-p53转染组p53表达水平提高18.5%—35.4%。与单纯AdCMV-p53转染组和单纯辐射组相比,辐射联合AdCMV-p53转染组细胞存活率分别降低25%—64%和22%—65%。To determine whether low-dose pre-irradiation could enhance adenovirus and expression in human prostate adenocarcinoma, the PC-3 cells were pre-exposed -mediated p53 transfer to y rays, and then infected with replication-deficient adenovirus recombinant vectors, containing human wild-type p53 （AdCMV-p53） or green fluorescent protein gene （AdCMV-GFP） respectively （y-ray irradiation ＋ AdC- MV-p53 /GFP infection）. The exogenous gene transfer and expression were detected by flow cytometric analysis. The GFP transfer frequencies in y irradiation ＋ AdCMV-GFP infection groups were 7%-39% more than those in AdCMV-GFP infection groups. The p53 levels in the γ irradiation ＋ AdCMV-p53 infection groups were 18. 5%-35.4% more than those in AdCMV-p53 infection groups （p〈0.05）, suggesting that low-dose （less than or equal to 1.0 Gy） irradiation could significantly promote exogenous p53 transfer and expression in the PC-3 cells. The survival fractions for the γ irradiation ＋ AdCMV-p53 infection groups were 25%-64%, 22%-65% less than those for AdCMV-p53 infection, or γ-irradiation groups, respectively （p〈0.05）.

关 键 词：P53基因 辐射 腺病毒载体 前列腺癌 辐射敏感性 

分 类 号：Q256[生物学—细胞生物学] Q279