金荞麦饮片液相色谱含量测定及指纹图谱研究  

Studies on HPLC-fingerprint of Rhizoma Fagopyri Dibotoryis

作　　者：杨滨[1] 辛敏通[1] 吕冬梅[1] 屈巧玲[1] 李化[1] 黄璐琦[1] 

机构地区：[1]中国中医科学院中药研究所,北京100700

出　　处：《中国中药杂志》2007年第12期1171-1174,共4页China Journal of Chinese Materia Medica

基　　金：北京市科技新星计划资助项目(H020821410190);中国博士后科学基金资助(2004035072)

摘　　要：目的:研究金荞麦饮片高效液相色谱指纹图谱及含量测定方法。方法:采用高效液相色谱-电化学检测法,Diamonsil C18(4.6 mm×250 mm,5μm)色谱柱;流动相为甲醇-0.1 mol.L-1磷酸盐缓冲液(pH 2.5);流速1.0 mL.min-1;柱温35℃;参比电极为ISAAC(in-situ Ag/AgC l);工作电极为玻碳电极;辅助电极为铂电极。结果:建立了金荞麦饮片高效液相色谱图谱,发现了6个色谱峰为共有峰,其中4个分别为没食子酸,原儿茶酸,原儿茶醛和表儿茶素的色谱峰。原儿茶酸的质量分数为0.004%～0.05%,原儿茶醛的质量分数为0.003%～0.015%。结论:该方法可用于金荞麦饮片质量控制。To establish an HPLC fingerprint of Rhizoma fagopyri dibotoryis. Method： The HPLC-electrochemical detection assay was used to establish the fingerprint of Rhizoma fagopyri dibotoryis. The sample was performed on a column of Diamonsil C18 （4. 6 mm×250 mm, 5μm） which was eluted with methanol-0.1 mol · L^-1 phosphate buffer （pH 2.5）, the flow rate was 1.0 mL · min^-1, the column temperature was 35℃, the reference electrode was ISAAC （in-situ silver/silver chloride）, the work electrode was glassy carbon, the counter electrode was Pt platmun. Result： The HPLC fingerprint profiles of 6 Rhizoma fagopyri dibotoryis contains 6 common chromatographic peaks, and gallic acid, protocatechuic acid, protocatechuic aldehyde and （ - ）-epicatechin were tested the samples. The contents of protocatechuic acid and protocatechuic aldehyde were from 0. 004% to 0. 05% and from 0. 003% to 0. 015%, respectively. Conclusion： The method can be used to control the quality of Rhizoma fagopyri dibotoryis.

关 键 词：金荞麦饮片 高效液相色谱-电化学检测 含量测定 指纹图谱 

分 类 号：R284.1[医药卫生—中药学]