Aspergillus ficuum菊粉酶的分离纯化与鉴定  

Purification and Identification of Aspergillus ficuum Inulinases

作　　者：王静[1] 金征宇[1] 江波[1] 徐学明[1] 

机构地区：[1]江南大学食品学院,江苏无锡214036

出　　处：《食品科学》2007年第6期188-192,共5页Food Science

基　　金：国家自然科学基金项目(20376029);教育部科学技术研究重点项目(104258)

摘　　要：Aspergillus ficuum菊粉酶系经硫酸铵分级沉淀、透析脱盐、DEAE-Cellulose离子交换色谱、Sepharose CL-6B凝胶过滤色谱和制备电泳技术分离纯化,获得五个电泳纯酶:三个外切菊粉酶组分Exo-I、Exo-II、Exo-III和两个内切菊粉酶组分Endo-I、Endo-II。SDS-PAGE分析测得五种酶的分子量分别为:Exo-I:69961Da,Exo-II:39973Da,Exo-III:45561Da,Endo-I:33574Da,Endo-II:30769Da。Aspergillusficuum inulinases were purified in a sequence of operations including ammonium sulfate fractionation, ion exchange chromatography on DEAE-Cellulose, column chromatography with Sepharose CL-6B and preparative electrophoresis. Five purified enzymes were thus obtained, two having an endo-type activity and the other being exo-actings. The five enzymes were identified as Exo-Ⅰ, Exo-Ⅱ, Exo-Ⅲ, Endo-Ⅰ and Endo-Ⅱ, with MW（molecular weight） of 69961Da, 39973Da, 45561Da, 33574Da, 30769Da, respectively, determined by SDS-PAGE.

关 键 词：菊粉酶 分离纯化 鉴定 ASPERGILLUS ficuum 

分 类 号：TS201.25[轻工技术与工程—食品科学]