化瘀通络中药对肾衰大鼠肾组织CTGF蛋白表达的实验研究  

Effects of Chinese Herbs with Functions of Removing Blood Stasis and Channel Obstruction on Expression of CTGF Protein in Renal Tissues of Rats with Renal Failure

作　　者：尹智炜[1] 陈志强[1] 丁奇峰[1] 张再康[1] 张芬芳[1] 杨阳[1] 

机构地区：[1]河北医科大学中西医结合研究所,河北石家庄050017

出　　处：《中国中医基础医学杂志》2007年第5期362-364,共3页JOURNAL OF BASIC CHINESE MEDICINE

基　　金：河北省博士资金项目(03547015D)

摘　　要：目的:采用蛋白质印迹法(Western Blot)检测化瘀通络中药肾络通对5/6肾切除肾衰大鼠残余肾结缔组织生长因子(CTGF)蛋白表达的影响。方法:5/6肾切除制备肾衰大鼠模型,随机分为假手术组、模型组、肾络通组和缬沙坦组,分别灌胃给药16周取材,应用Western Blot法检测肾组织CTGF蛋白的表达。结果:模型组与假手术相比,CTGF蛋白的表达明显增高;肾络通组CTGF蛋白表达较模型组显著降低。结论:肾络通可使慢性进展性纤维化肾组织CTGF蛋白表达下调,延缓5/6肾切除肾衰大鼠的病程进展,提示以CTGF为切入点,运用不同的有效中药组方对其加以研究,将为肾纤维化防治提供新的思路。Objective： To Analysis of CTGF＇s protein in rats＇ remnant kidney of 5/6 nephrectomy induced chronic renal failure （CRF） with treatment of traditional Chinese medicine of disperse blood stasis and dredge collateral Shen Luo Tong by Western Blot. Method： The CRF model of rat made through 5/6 nephrectomy. The rats were divided into four groups including Sham group, Model group, Shen Luo Tong group and Valsartan group. After 16-week intragastric administration, the expression of CTGF＇ s protein in nephridial tissue was detected by Western Blot. Results： Compare with Sham group, the expression of CTGF＇ s protein of Model group was increased obviously; While compare with Model group, the expression of CTGF＇ s protein of Shen Luo Tong and Valsartan group was decreased obviously. Conclusion： Shen Luo Tong maybe play an great role in delaying the proceeding of CRF by down-regulation the expression of CTGF＇ s protein. It indicated that study of down-regulation the expression of CTGF with treatment of different traditional Chinese medicine compound recipe will provide new methods for the prevention and cure of renal fibrosis.

关 键 词：化瘀通络 肾络通 结缔组织生长因子 Western BLOT 

分 类 号：R285.5[医药卫生—中药学]