视网膜色素上皮细胞凋亡途径的研究  

Study on apoptotic pathway of retinal pigment epithelial cells

作　　者：庞东渤[1] 洪晶[1] 

机构地区：[1]辽宁医学院附属第一医院眼科,辽宁省锦州市121001

出　　处：《眼科新进展》2007年第6期405-407,共3页Recent Advances in Ophthalmology

基　　金：国家自然科学基金资助(编号:30471865)~~

摘　　要：目的研究维拉帕米(verapamil,Ver)诱导体外培养的人视网膜色素上皮(reti-nalpigmentepithelium,RPE)细胞凋亡过程的凋亡途径。方法应用80mg·L-1Ver作用体外培养的人RPE细胞12h、24h、48h诱导凋亡。RT-PCR及Western blot检测caspase-3及caspase-8表达。结果对照组RPE细胞可见caspase-3的mRNA及蛋白有少量的表达,Ver作用12h、24h、48h时,caspase-3的mRNA及蛋白表达增强。对照组和Ver作用12h、24h、48h组caspase-3与内对照β-actinRT-PCR产物OD比值的平均值分别为0.58±0.08、0.89±0.12、1.22±0.14及1.18±0.09,组间比较,差异有显著意义(F=19.22,P<0.05)。本实验条件下未发现caspase-8的mRNA及蛋白表达。结论Ver诱导体外培养人RPE细胞凋亡可能是通过细胞内途径完成的。Objective To explore the apoptotic pathway of cultrured human retinal pigment epithelium （RPE） cells apoptosis induced by verapamil. Methods The apoptosis of cultrured human RPE cells was induced by 80 mg · L^-1 verapamil for 12 hours,24 hours,48 hours. RT-PCR and Western blot were used to determine the expression of caspase-3 and caspase-8. Results The expression of caspase-3 mRNA and protein was slight in normal RPE cells and increased after cocultured with verapamil for 12 hours,24 hours and 48 hours.The mean OD ratio of caspase-3 and B-actin in each group were 0.58 ± 0.08,0.89 ± 0. 12,1.22 ±0.14 and 1. 18 ±0.09.There was significant difference between each group（ F = 19.22,P 〈0.05 ）. Under our experimental conditions,the expression of caspase-8 mRNA and protein weren＇ t detected. Conclusion Apoptosis of cultured human RPE cells induced by verapamil might be accomplished through intracellular pathway.

关 键 词：维拉帕米 视网膜色素上皮 细胞凋亡 半胱氨酸天冬氨酸蛋白酶 

分 类 号：R774.1[医药卫生—眼科]