茶多酚锰合成、表征及络合和诱导肿瘤细胞凋亡的研究  

Synthesis,Characterization and Binding of Tea Polyphenol-Manganese and Apoptosis Rate of Induced Tumor Cells

作　　者：黄河宁[1] 李安章[1] 翁露娜[1] 林庆梅[2] 黄河清[1] 郑忠辉[1] 李棋福[1] 蔡宗苇[3] 

机构地区：[1]厦门大学生命科学学院细胞生物学与肿瘤细胞工程教育部重点实验室 [2]厦门大学海洋与环境学院环境科学研究中心,厦门361005 [3]香港浸会大学化学系

出　　处：《高等学校化学学报》2007年第6期1072-1076,共5页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金(批准号:30470372);厦门市科技项目(批准号:3502Z2001263);三明市科技项目(批准号:2004-L-1)资助

摘　　要：合成了茶多酚锰(TP-Mn)和茶多酚锗(TP-Ge),并采用红外光谱法及液相色谱质谱联用技术表征TP-Mn的理化特性.选用反向液相色谱(RP-HPLC)和电感藕合等离子体质谱(ICP-MS)技术研究牛血清白蛋白(BSA)络合TP-Mn的能力,指出BSA可直接络合于TP-Mn.采用MTT法研究TP和TP-Mn诱导Hela卵巢癌细胞的凋亡率.结果表明,TP和TP-Mn均能诱导Hela卵巢癌细胞凋亡,但TP-Mn诱导凋亡率约为TP的2倍.比较TP-Ge和TP-Mn诱导Raji人B淋巴瘤细胞凋亡率发现,两者诱导肿瘤细胞的凋亡率几乎相同,均高达86%左右.人血清白蛋白(HSB)可作为输送TP-Ge和TP-Mn的药物载体.Both tea polyphenol-manganese（TP-Mn） and germanium （TP-Ge） were synthesized with organic synthesis approaches. The characterizations of TP-Mn were clearly revealed via infrared spectrometry and HPLC-ESI-Q-TOF mass spectrometry, respectively. Both HPLC and ICP-MS methods were employed to study the capacities for bovine serum albumen （ BSA ） binding to TP-Mn, indicating these capacities feasibility. Apoptosis rates in Hela ovarian induced with TP and TP-Mn were studied by a 3-（4,5-dimethyhhiazol-2-yl）-2,5-diphenly tetrazolium bromide（MTT） method. The experimental results show that both TP and TP-Mn can induced the cancer cell apoptosis, but the apoptosis rate with TP-Mn showed two times higher than that with TP. As compared experiments, the same apoptosis rates, going with up to approximately 86% , in Raji human B lymphoma induced with both TP and TP-Mn were observed directly. Human serum albumen might be employed to be a pharmacy carrier for transferring TP-Mn and TP-Ge in vivo.

关 键 词：茶多酚锰 茶多酚锗 肿瘤细胞 凋亡率 电感藕合等离子体质谱 

分 类 号：O629.7[理学—有机化学]