新的玉米显性矮秆基因的发现及初步分析  

Discovery of a New Dominant Dwarf Gene in Maize and Its Preliminary Study

作　　者：张素梅[1] 刘凤军 刘保申[1] 王立静[1] 董树亭[1] 

机构地区：[1]山东农业大学农学院,山东泰安271018 [2]禹城市农业局,山东禹城251200

出　　处：《玉米科学》2007年第3期15-18,共4页Journal of Maize Sciences

摘　　要：玉米杂交种CL1077中的矮秆突变体52333与5个高秆自交系进行杂交,对P1、P2、F1、F2、BC1和BC2群体株高变异进行分析。结果表明,正反交杂种F1均表现为矮秆,没有显著差异;F2代矮株与高株的分离比为3∶1,杂种F1与高株自交系回交后代的分离比为1∶1,与矮秆突变体回交的后代全为矮株,证明该矮秆材料的矮秆性状受一对显性矮秆基因控制,且不受细胞质的影响。对纯合矮秆植株及杂种F1芽期和苗期进行赤霉素处理,结果表明,此矮秆基因对赤霉素敏感,表明与以前报道的所有矮秆基因不同,此矮秆基因可能是一新的矮秆基因,并将此矮秆基因初步定名为D(t)。Based on the analysis of the population variations of plant height in hybrids including P1, P2, F1, F2, BC1 （backcrossed to dwarf parent） and BC2 （backcrossed to tall parents） derived from five cross combinations between a dwarf mutant of 52333 and tall inbred lines, the F1 individuals were as short as the short parent, and having no notable differences in contrast to control, and the segregation of the F2 generation appeared to be 3 ： 1, the dwarf F1 hybrids with the new dwarf gene were backcrossed with the tall inbred lines, the approximately segregation ratio of BC1 populations was 1 ： 1, and the off springs derived from that the combination of F1 with 52333, were all dwarf plants, so the dwarfing effect of this new gene was confirmed, indicated that the dwarf trait of 52333 was controlled by one dominant gene and its expression could not be affected by cytoplasm. Treating the pure dwarf plant and hybrid F1 with exogenous gibberellic acid at the sprout and seedling stages, the result proved that the dwarf gene was sensitive to gibberellin, so the dwarf gene maybe a new dwarf gene, and named it as D（t）.

关 键 词：玉米 矮秆基因 突变体 赤霉素 

分 类 号：S513.024[农业科学—作物学]