低钾胁迫对玉米自交系苗期部分信号分子的影响  

The Effect of Partial Signaling Molecule of Inbred Lines in Maize Seedling Under Low Potassium Stress

作　　者：刘延吉[1] 田晓艳[2] 曹敏建[1] 

机构地区：[1]沈阳农业大学生物科学技术学院,沈阳110161 [2]辽宁石油化工大学环境与生物工程学院,辽宁抚顺113001

出　　处：《玉米科学》2007年第3期87-90,94,共5页Journal of Maize Sciences

基　　金：国家重点基础研究发展计划(G1999011707)

摘　　要：研究在低钾胁迫下玉米苗期胞间和胞内部分信号分子的改变。玉米自交系A(不耐低钾)和B(耐低钾),以1/2Hoagland溶液培养12d后,转入3个不同钾离子浓度(5μmol/L,100μmol/L,1000μmol/L)连续培养50d,测定其胞间ABA,IAA,GA,ZR激素水平及胞内CaM含量、PMCa2+-ATPase和NADK激酶活性。结果表明:低钾条件下,ABA/GA、ABA/IAA、ABA/ZR比值上升;同时,耐性品种CaM含量增加;低钾诱导了PMCa2+-ATPase和NADK响应,耐性品种的PMCa2+-ATPase和NADK活性高于非耐性品种。低钾条件下,(耐低钾自交系B通过胞间ABA/GA、A-BA/IAA、ABA/ZR比值变化,促进胞内CaM含量升高,导致下游PMCa2+-ATPase和NADK激酶活性升高),通过改变胞内Ca2+和NADP/NAD水平,适应低钾胁迫。The article studied the change of intercellular and intracellular partial signaling molecule in maize seedling under low potassium stress. Two inbred lines of maize, B which was tolerant to K^＋ deficiency and A which was sensitive to K^＋ deficiency, were cultured in 1/2Hoagland nutrient solutions for 12 days, then transplanted them into three different K^＋ concentration （5 μmol/L, 100 μmol/L, 1 000 μmol/L） to continue cultured for 50 days. Assayed intercellular hormone content of ABA, IAA, GA, ZR and intracellular enzyme activity of PMCa^2＋-ATPase and NADK, and also assay intracellular CaM content. The results showed that the ratio of ABA/GA, ABA/IAA and ABA/ZR increased, the CaM content of B increased under low K^＋ concentration. It induced response of PMCa^2＋-ATPase and NADK, these activities of ABA/GA, ABA/IAA and ABA/ZR of B .were higher than those of A under low potassium stress. The results indicated that the ratio change of ABA/GA, ABA/IAA and ABA/ZR of intercellular signal improved intracellular CaM content, induced PMCa^2＋-ATPase and NADK activities of downstream to increase. The increasing activities of PMCa^2＋-ATPase and NADK influenced the content of Ca^2＋ and NADP/NAD in order to be adapted to low potassium stress.

关 键 词：玉米 低钾胁迫 钙调素 内源激素 NAD激酶 质膜Ca^2+-ATP酶 

分 类 号：S513.01[农业科学—作物学]