丹参注射液在大鼠肝脏热缺血再灌注损伤中的保护作用  

The Protective and Therapeutic Effects of Radix Salviae Miltiorrhizae(RSM)in Warm Ischemia-Reperfusion Injury of the Murine Liver

作　　者：吕克之[1] 杨卫平[1] 张明钧[1] 蔡伟耀[1] 李宏为[1] 

机构地区：[1]上海交通大学医学院附属瑞金医院外科上海消化外科研究所,上海200025

出　　处：《外科理论与实践》2007年第3期264-267,共4页Journal of Surgery Concepts & Practice

摘　　要：目的:对中成药丹参(RSM)注射液在大鼠肝缺血再灌注损伤(WI/RI)的保护作用机制作了初步研究。方法:采用二次阻断法建立热缺血再灌注损伤的动物模型,观察对照组与丹参组,手术后24小时内各时点的肝功能生化指标ALT、AST的变化情况并应用内参照定量逆转录聚合酶链反应技术(Q-RT-PCR)分析两组间肝脏组织中TNF-α在不同时点的表达变化情况。结果:丹参组和对照组各时点在肝脏生化指标均存在显著差异。丹参组肝脏组织内TNF-α表达明显降低且早于肝功能生化指标的变化。结论:丹参具有防治缺血再灌注损伤的作用,其机制之一是通过降低TNF-α表达并阻断由此引发的一系列级联反应的机理来实现的。Objective To study the protective and therapeutic effects of Radix Salviae Miltiorrhizae （RSM）, a kind of famous Chinese herb, in treating warm ischemia-reperfusion injury （WI/RI） of the rat liver. Methods An animal model of modified partial hepatic ischemia-reperfusion injury was established in the rat by blocking twice the blood inflow of the liver. The male Sprague-Dawley rats （n=70） were then divided into two groups at random, Group 1 （Control group n=35） and Group 2 （RSM group n =35）. The methods of biochemistry analysis and quantitative reverse transcriptase polymerase chain reaction （Q-RT-PCR） were used to determine the serum levels of alanine aminotransferase （ALT） aspartate, aminotransferase （AST） and the expression of tumor necrosis factor-or （TNF-α） 0, 1, 3, 6, 9, 12 and 24 hours after the rats were submitted to 100 min of WI/RI. Results The ALT, AST and TNF-α showed significant differences （P〈0.05＆ P〈 0.01） between the RSM and control groups in almost all parameters. The intrahepatic TNF-α also showed remarkable difference between the 2 groups at 0,1,3,9 and 12 hours. At the same time, we also found the expression of TNF-α lowered immediately after reperfusion, much faster than the speed of the ALT and AST. Conclusions WI/RI can cause severe hepatocyte damage, and RSM could prevent and treat WI/RI effectively. The mechanisms may lie in the improvement local microcirculation, anti-inflammation, inhibition of TNF-α expression and other cytokines＇ cascade as well. Rats

关 键 词：丹参 再灌注损伤 肿瘤坏死因子 大鼠 

分 类 号：R285.5[医药卫生—中药学]