磷酸化对多肽中Xaa-Pro片段肽脯酰胺键顺反异构的影响研究(英文)  

Effect of Phosphorylation on Peptidyl-Prolyl Imide Bond cis/trans Isomerization of Peptides With Xaa-Pro Motif

作　　者：朱振泰[1] 孙命[2] 郭燕婷[1] 李艳梅[1] 

机构地区：[1]清华大学化学系,生命有机磷化学和化学生物学教育部重点实验室,北京100084 [2]中国科学院生物物理研究所,北京100101

出　　处：《生物化学与生物物理进展》2007年第6期585-594,共10页Progress In Biochemistry and Biophysics

基　　金：国家自然科学基金(20472041,20532020);国家教育部杰出青年教师及博士生高等教育研究基金(20030003049)资助项目~~

摘　　要：多肽和蛋白质中Xaa-Pro片段肽脯酰胺键顺反异构对其构象与功能有重要影响.设计合成了一系列模型多肽及其磷酸化多肽,并采用核磁共振实验和分子动力学模拟的方法,研究了所合成多肽中肽脯酰胺键的顺反异构化.结果表明,对脯氨酸之前的Xaa残基进行侧链O-磷酸化会极大地影响该顺反异构化过程,进而调节肽链构象.此外,磷酸化使得多肽顺式构象比例增加,且当磷酸基团不带负电荷时顺式构象所占比例最大.同时,分子动力学模拟所得结果与核磁共振实验相一致,包括最稳定构象和顺反构象统计分布.磷酸基团所带电荷及其空间位阻可能是影响这类磷酸化多肽构象变化的主要因素.The peptidyl-prolyl imide bond cis/trans isomerization of Xaa-Pro motif in the peptide and protein plays an important role to influence their conformation and function. Here, a series of model peptides including phosphorylated and its unphosphorylated counterparts were designed and synthesized. Preliminary ^1H NMR experiments and molecular dynamics （MD） simulation were used to analyze the peptidyl-prolyl cis/trans imide bond isomerization. The data indicated that the side-chain O-phosphorylation of the Xaa residues preceding proline affected evidently the isomerization and thereby regulated the peptides conformations. The charges of the phosphate moiety as well as their steric effects might be the driving force for the conformational changes of these phosphopeptides. Moreover, the obtained most stable multiple configurations and their statistic cis/trans concentration distribution in MD simulation were basically consistent with the NMR experiments, which demonstrated that phosphorylation increased the cis conformation of the peptide and the maximum cis ratio is given while the phosphate group has no negative charge.

关 键 词：肽脯酰胺键 异构化 磷酸化 顺反异构比 分子动力学模拟 

分 类 号：O62[理学—有机化学]