hCDC14A与BRAP2相互作用的初步研究  

Primary analysis on interaction between hCDC14A and BRAP2

作　　者：胡海英[1] 张烁[1] 何敏[1] 胡仁明[1] 

机构地区：[1]复旦大学附属华山医院内分泌科复旦大学内分泌糖尿病研究所,上海200040

出　　处：《中国病理生理杂志》2007年第6期1041-1044,共4页Chinese Journal of Pathophysiology

基　　金：国家"973"子课题资助项目(No.2002CB713703);国家自然科学基金重点资助项目(No.30230380)

摘　　要：目的:筛选hCDC14A(human cell division cycle gene14A)的相互作用蛋白,探索功能联系。方法:酵母双杂交筛选hCDC14A的可能相互作用蛋白,Pulldown实验和免疫共沉淀实验验证蛋白质间相互作用,免疫荧光显微镜观察目的基因在细胞中的表达和定位,体内泛素化实验提示可能的相互作用机制。结果:酵母双杂交筛查到BRAP2(BRCA1 associated protein2)为hCDC14A的可能相互作用蛋白,两者有共同定位,BRAP2可以增强hCDC14A的泛素化修饰。结论:BRAP2可以与hCDC14A相互作用,可能是hCDC14A的泛素连接酶。AIM： To search for new candidate partners of human cell division cycle gene 14A （hCDC14A） and explore their functional relationships. METHODS ： Yeast two - hybrid screening was employed to find hCDC14A new parthers. Physical interaction between two proteins was verified using Pulldown and co - immunoprecipitation assays. Subeellular localizations were revealed by immunofluorescence. In vivo ubiquitination test implied their potential functional relationship. RESULTS： BRAP2 （BRCA1 associated protein 2） was found to be a new candidate partner of hCDC14A, hCDC14A was modified by ubiquitination, and BRAF2 increased this modification in vivo. As expected, hCDC14A and BRAF2 co - localized on mitotic spindles in HeLa cells. CONCLUSION： BRAP2 may be an ubiquitin E3 ligase of hCDC14A.

关 键 词：基因 CDC14A 酵母双杂交 泛素连接酶 BRCA1 相关蛋白质2 

分 类 号：Q253[生物学—细胞生物学]