非标记电化学DNA杂交指示剂  

The Non-Labeling Electrochemical Active Deoxyribonucleic Acid Hybridization Indicators

作　　者：汪庆祥[1] 袁显龙[1] 焦奎[1] 谢江坤[1] 张波[1] 

机构地区：[1]青岛科技大学化学与分子工程学院,青岛266042

出　　处：《化学进展》2007年第6期1007-1015,共9页Progress in Chemistry

基　　金：国家自然科学基金项目(20635020)资助

摘　　要：电化学DNA杂交检测技术因其快速、灵敏、低消耗和易于操作等优点而在临床医学、环境监测和药物分析等领域受到普遍关注。电化学DNA杂交指示剂是DNA电化学杂交传感器的重要组成部分,能与单链DNA和双链DNA通过不同的模式和作用力进行差异性结合。本文介绍了电化学杂交指示剂的定义及其在DNA电化学传感器中的重要性,根据分子结构上的特征差异,将非标记型电化学杂交指示剂分为有机染料(荧光素)、药物分子和金属配合物三类,并从中选取了各个类型中具有代表性的指示剂对它们的工作原理、研究进展和应用现状进行了比较和评述。对杂交指示剂的设计和开发前景,特别是满足基因芯片应用等方面做出了展望。The electrochemical detection of DNA hybridization has received much attention in the fields of clinical diagnosis, environmental monitoring and drug analysis due to its high sensitivity, rapid response, easy handling, and low cost. The non-labeling electrochemical active DNA hybridization indicators, a series of electrochemical active small molecules that bind to single-stranded and double-stranded DNA via different binding modes and action force, are important components of electrochemical DNA biosensor. The concepts of the non-labeling electrochemical hybridization indicators and their significance in DNA biosensors arc introduced. According to the differences of the molecular structure characteristic, the electrochemical hybridization indicators are classified into organic dyes, drugs and metal complexes. The recent research and application of representative indicators in each species are reviewed in detail. The designation, exploitation and the application of new indicators are prospected, especially for the development of gene chip technologies.

关 键 词：DNA 杂交指示剂 电化学活性 

分 类 号：O657.1[理学—分析化学] TP212.2[理学—化学]