人骨髓间充质干细胞在体内外分化为心血管组织  

Differentiation of human marrow mesenchymal stem cell into cardiovascular tissue in vitro and in vivo

作　　者：刘煜昊[1] 房佰俊[2] 史明霞[2] 杨少光[2] 廖联明[2] 赵春华[2] 

机构地区：[1]河南省人民医院心血管内科,河南郑州450003 [2]中国医学科学院中国协和医院大学基础医学研究所组织工程中心,北京100005

出　　处：《基础医学与临床》2007年第6期643-647,共5页Basic and Clinical Medicine

摘　　要：目的验证人骨髓间充质干细胞(MSC)分化为心血管组织的潜能。方法用5-氮胞杂苷诱导MSC向心肌细胞分化,用VEGF-B诱导向血管内皮细胞分化。异丙肾上腺素法制成NOD/SCID小鼠心肌损伤模型,经尾静脉注入标记的MSC。免疫荧光法检测MSC的体内、外分化。结果在体外,经诱导的MSC表达肌凝蛋白重链和肌钙蛋白I,表达Ⅷ因子相关抗原和CD31。在体内,标记的MSC表达阳性的肌凝蛋白重链和Ⅷ因子相关抗原。结论MSC可分化为心肌和血管内皮细胞,是再生医学的理想种子细胞。Objective To test the potential of human marrow mesenchymal stem cells （MSCs） differentiating into cardiovascular tissue. Methods 5-azacytidine （5-aza） was used to induce MSCs ＇ s differentiating into myocardial cells. MSCs were induced to differentiate into endothelial cells by VEGF-B. Isoproterenol was used to canse myo- cardial injury model in NOD/SCID mice. Labeled MSCs were injected into tail vein. Immunofluorescence was performed to evaluate MSCs differentiation in vitro and vivo. Results MSCs treated by 5-aza expressed cardiac myosin heavy chain and cardiac troponin I in vitro. MSCs induced by VEGF-B expressed yon Willebrand factor （vWF） and CD31 in vitro. MSCs implanted in myocardium were positively stained for cardiac myosin heavy chain and vWF. Condusion MSCs can differentiate into myocardial cell and endothelial cell, so they are ideal seed cells in regeneration medicine.

关 键 词：骨髓间充质干细胞 分化 心肌细胞 血管内皮细胞 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]