抗白介素5单克隆抗体抑制哮喘小鼠嗜酸性粒细胞迁移  

Anti-IL-5 monoclony antibody inhibits the migration of eosinophil in a murine model of asthma

作　　者：江荣林[1] 沈华浩[2] 王绍斌[2] 王萍莉[2] 马伟斌[1] 

机构地区：[1]浙江省中医院ICU,浙江杭州310006 [2]浙江大学医学院第二附属医院呼吸科,浙江杭州310006

出　　处：《基础医学与临床》2007年第5期539-543,共5页Basic and Clinical Medicine

摘　　要：目的观察抗白介素5(IL-5)单克隆抗体对哮喘小鼠嗜酸性粒细胞(Eos)从骨髓到气道迁移的影响。方法15只雄性C57BL/6小鼠。常规法复制哮喘,并在每次卵白蛋白(Ova)激发前2h鼻腔内滴入抗IL-5单克隆抗体,同时设立阴性对照组,即以生理盐水代替Ova和抗IL-5抗体。在末次抗原激发后12h测定支气管肺泡灌洗液(BALF)、外周血(PB)和骨髓(BM)中炎症细胞数以及肺组织内Eos数。结果Ova抗原激发后12h小鼠的BALF、PB和BM及细支气管和肺组织中的Eos数显著增加(P<0·01,P<0·05)。抗IL-5单克隆抗体则使上述变化显著减轻(P<0·05,P<0·01)。结论抗IL-5单克隆抗体能显著抑制哮喘小鼠嗜酸性粒细胞的迁移。Objective To study the effect of anti-IL-5 monoclony antibody （TRFK-5） on migration of Eos from BM to the airways in sensitized mice. Methods Male C57BL/6 （6-8wk of age） murine model of Asthma and control group were estabolished with routine method. The outcome measurements include white blood cell （WBC） total count, differential count of bronchoalveolar lavage fluid （BALF） and peripheral blood （ PB）, nuclear cell count and eosinophil percentage of BM. These parameters were collected 12 h after the final allergen challenge. To cheek Eos infifltration, the histology of lung tissues was also observed. Further, the effects of intranasal TRFK-5 on above changes were investigated. Results Eosinophil numbers of BALF, PB, BM and the infiltration of Eos in pulmovnary tissues were increased considerably 12 h after final OVA challenge compared with negative group （P 〈0. 01, P 〈0. 05）. TRFK-5 decreases the above changes significantly（ P 〈 0. 05, P 〈 0. 01 ）. Conclusion TRFK-5 inhibits the migration of eosinophil in a murine model of Asthma.

关 键 词：哮喘 动物模型 嗜酸性粒细胞 抗IL-5单克隆抗体 

分 类 号：R562.25[医药卫生—呼吸系统]