小麦抗白粉病基因Pm6的微卫星标记鉴定  

Identification of a microsatellite marker linked with powdery mildew resistance gene Pm6 in wheat

作　　者：王俊美[1] 刘红彦[1] 王飞[1] 康振生[2] 段双科[2] 

机构地区：[1]河南省农业科学院植物保护研究所,郑州450002 [2]西北农林科技大学植保资源与病虫害治理教育部重点开放实验室,杨凌712100

出　　处：《植物病理学报》2007年第3期329-332,共4页Acta Phytopathologica Sinica

基　　金：河南省杰出青年基金(04120001400);西北农林科技大学植保资源与病虫害治理教育部重点开放实验室资助项目;教育部长江学者和创新团队发展计划项目(IRT0558)

摘　　要：Wheat powdery mildew, caused by Blumeria graminis f. sp. tritici, is a prevalent disease worldwide. Breeding and planting resistance cultivars have been proved effective and environmental friendly for control of the disease. To develop easily used PCR-based markers in marker assisted selection （MAS） for Pm6, a dominant powdery mildew resistance gene in wheat, 25 microsatellite markers on chromosome 2BL in wheat were screened between susceptible parent Yumai13 and resistance parent Timgalen carrying Pm6. F2 population derived from Yumai13 and Timgalen was further analyzed by the marker Xgwm501. The results indicated that Xgwm501 was a co-dominant marker linked to Pm6 gene at a distance of 14.8 cM. 29 Pm-carrying varieties were tested by the marker Xgwm501 and only those carrying Pm6 showed 117 bp resistance specific band. This marker is proved to have high practicability and can be used in MAS of Pm6 gene in wheat breeding programs.Wheat powdery mildew, caused by Blumeria graminis f. sp. tritici, is a prevalent disease world- wide. Breeding and planting resistance cultivars have been proved effective and environmental friendly for control of the disease. To develop easily used PCR-based markers in marker assisted selection （MAS） for Prn6, a dominant powdery mildew resistance gene in wheat, 25 microsatellite markers on chromosome 2BL in wheat were screened between susceptible parent Yumail3 and resistance parent Timgalen carrying Prn6. F2 population derived from Yumail3 and Timgalen was further analyzed by the marker Xgwm501. The results indicated that Xgwm501 was a co-dominant marker linked to Prn6 gene at a distance of 14.8 cM. 29 Pro-carrying varieties were tested by the marker Xgwm501 and only those carrying Pro6 showed 117 bp resistance specific band. This marker is proved to have high practicability and can be used in MAS of Prn6 gene in wheat breeding programs.

关 键 词：抗白粉病基因 小麦白粉病 Pm6 标记鉴定 微卫星 提莫菲维小麦 小麦病害 抗病基因 

分 类 号：S512.1[农业科学—作物学]