饮食诱导肥胖易感和肥胖抵抗大鼠HSL和LPL基因表达的研究  

Gene expression of hormone sensitive lipase and lipoprotein lipase in obesity-prone and obesity-resistant rats induced by high-fat diet

作　　者：麻微微[1] 赵丹[1] 苏畅[1] 王舒然[1] 蔺威鸣[1] 王国秀[1] 

机构地区：[1]哈尔滨医科大学公共卫生学院营养与食品卫生教研室,哈尔滨150081

出　　处：《卫生研究》2007年第3期320-322,共3页Journal of Hygiene Research

基　　金：国家自然科学基金资助项目(No.30300283)

摘　　要：目的探讨高脂饮食诱导肥胖易感(OP)大鼠和肥胖抵抗(OR)大鼠,激素敏感性脂肪酶(HSL)和脂蛋白脂肪酶(LPL)基因表达的差别。方法健康雄性SD大鼠80只,高脂饲料喂饲5周后,筛选出OP大鼠和OR大鼠,再将所有大鼠转为基础饲料喂饲10周后,处死动物,收集血清和白色脂肪组织,应用RT-PCR方法比较白色脂肪组织HSL和LPL基因的表达。结果高脂饲料喂饲5周后,OP大鼠白色脂肪组织HSL基因表达显著低于OR大鼠,而LPL基因表达显著高于OR大鼠;而转成基础饲料喂饲10后,OP大鼠HSL基因表达与OR大鼠无显著差异,但LPL基因表达仍显著高于OR大鼠。结论HSL基因表达水平下降和LPL基因表达水平升高,通过抑制脂肪分解促进脂肪合成,导致高脂饮食诱导肥胖易感大鼠的形成。HSL基因高表达更多是由高脂饮食诱导产生的,而LPL基因表达升高才是肥胖大鼠的特征性基因表达改变。To explore the gene expression of hormone sensitive lipase （HSL） and lipoprotein lipase （LPL） between obesity-prone （OP） and obesity-resistant （OR） rats induced by a high-fat diet. Methods Eighty male SpragueDawley （SD） rats were fed with a high-fat diet for 5 weeks, then OP and OR rats were selected according to their body weight gain. All the rats were fed with basic diet for another 10 weeks, the rats were then sacrificed, and serum samples were collected. To discuss the function of HSL and LPL during the formation of obesity or obesity resistance, RT-PCR was used to compare the gene expression of HSL and LPL in white adipose tissue. Results At the end of the 5th week, when compared with OR rats, the gene expression of HSL in white adipose tissue was lower in OP rats, but the gene expression of LPL was higher in OP rats. After switched to basic diet for 10 weeks, HSL gene expression had no difference between OP and OR rats, but LPL gene was still highly expressed in OP rats. Conclusion The formation of obesity prone rats may be associated with decreased HSL gene expression and increased LPL gene expression, which promote adipose synthesis and inhibit lipolysis. Highly expressed HSL gene may be associated with high fat diet, while increased LPL gene expression is the specific change of OP rats.

关 键 词：肥胖易感 肥胖抵抗 脂蛋白脂肪酶 激素敏感性脂肪酶 基因表达 

分 类 号：R589.2[医药卫生—内分泌] R155.1[医药卫生—内科学]