角类肥蛛体躯不同部位酯酶同工酶的比较分析  

Differences in Esterase Isozymes in Different Parts of Lariniodes cornuta

作　　者：程圆圆[1] 陈建[1] 刘凤想[1] 

机构地区：[1]湖北大学生命科学学院,武汉430062

出　　处：《动物学杂志》2007年第3期97-101,共5页Chinese Journal of Zoology

基　　金：国家自然科学基金项目(No.30370206)

摘　　要：运用聚丙烯酰胺凝胶电泳的方法对角类肥蛛(Lariniodes cornuta)头胸部和腹部的酯酶同工酶酶谱进行了比较分析。结果表明,角类肥蛛的酯酶是单体酶,头胸部和腹部的酯酶酶谱差异显著。腹部的酯酶呈现4个位点:Est-1、Est-2、Est-3、Est-4。Est-1和Est-4位点为纯合基因型,Est-2和Est-3位点为杂合基因型。头胸部的酯酶仅表现出2个位点:Est-2和Est-3,且这2个位点是纯合基因型。不同个体之间头胸部的酯酶没有明显差异,Est-2b和Est-3a可以作为鉴别角类肥蛛的特征酶带;腹部的酯酶则存在明显的个体差异,在Est-2和Est-3位点的基因杂合度为h2=h3=0·4779。由此可见,酯酶同工酶可以作为角类肥蛛遗传变异的分子标记,是研究个体间遗传差异、居群的遗传结构以及种间进化关系的基础。The genetic structure of esterase colorimetric （EST） in cephalothorax and abdomen of Lariniodes cornuta was studied by using isozymc analysis with polyacryamide gel electrophoresis （PAGE）. As a result, EST in L. cornuta was a monomeric enzyme and there were some important differences between cephalothorax and abdomen in the EST zymogram. EST in abdomen presented Est-1, Est-2, Est-3, Est-4 loci. Est-1 and Est-4 were homozygote while the others （Est-2 and Est-3） were heterozygotc. However, EST in cephalothorax only stated two loci： Est-2 and Est-3. Both of them were homozygote. Furthermore, the EST zymogram of cephalothorax was alike in different individuals. And Est-2b and Est-3a could be used as the characteristic zymogram for differentiating L. cornuta. Nevertheless, some differences appeared in the abdomen zymogram of different individuals. The frequency of heterozygote （ hi ） for the Est-2 and Est-3 was 0.477 9. Besides we also discussed the significance of our findings for further studies on analyzing genetic variation in individuals and in populations and phylogenetic analysis.

关 键 词：角类肥蛛 酯酶同工酶 聚丙烯酰胺凝胶电泳 

分 类 号：Q55[生物学—生物化学]