hPer1相互作用蛋白的筛选  

Screening the Interacting Proteins of hPer1

作　　者：冀治鸿[1] 江舟[1] 汪宇辉[1] 鲁芳[1] 刘延友[1] 高梅[1] 王正荣[1] 

机构地区：[1]四川大学华西医学中心基础与法医学院生物医学工程研究室,四川成都610041

出　　处：《航天医学与医学工程》2007年第3期184-187,共4页Space Medicine & Medical Engineering

基　　金：国家自然科学基金资助项目(30470684;30570902)

摘　　要：目的应用酵母双杂交系统筛选血液中与人体生物钟蛋白Per1相互作用的新蛋白。方法以人Per1的basicHLH-PAS结构域为诱饵,在人血液cDNA文库中筛选与之相互作用的新蛋白。阳性克隆送测序后用生物信息学分析。结果酵母双杂交文库营养缺陷筛选得到114个阳性克隆,β-半乳糖苷酶检测报告基因获得46个蓝色克隆。通过测序和生物信息学分析,其中之一能与Per1相互作用的蛋白为LSMD1。结论通过酵母双杂交系统证明Per1能够与LSMD1发生相互作用。Objective To find a new method for identifying interacting proteins of human Perl in the blood. Methods Using bHLH-PAS domain of hPerl as bait, a human blood cDNA library was screened and the proteins interacting with hPerl were searched. The positive clones were sequenced and analyzed by bioinformatics methods. Results Forty-six colonies were selected by the yeast two-hybrid system. LSMDI protein was obtained after verification. Conclusion It is proved that Perl can interact with LSMD1 by yeast two-hybrid system.

关 键 词：近日节律 酵母双杂交 Per1基因 LSMD1蛋白 相互作用蛋白 

分 类 号：R852.6[医药卫生—航空、航天与航海医学]