重组IL-12对乙肝疫苗在小鼠诱导免疫应答的作用  

The Role of Recombinant IL-12 in Enhancing Immune Responses Induced by Hepatitis B Vaccine in Mice

作　　者：陆群[1] 柯金山 周丽霞[1] 赵延荣[1] 何 秦学亮 苗小光[1] 金洁[1] 

机构地区：[1]上海市中西医结合医院检验科,200082 [2]上海杨思医院检验科,200126

出　　处：《放射免疫学杂志》2007年第3期265-267,共3页Journal of Radioimmanology

摘　　要：目的:观察重组IL-12对乙肝疫苗在小鼠诱导应答的强度与性质的作用,探讨将重组IL-12用作乙肝治疗性疫苗分子佐剂的可能性。方法:将乙肝疫苗联合重组IL-12肌注免疫小鼠,检测小鼠产生的抗乙肝表面抗原特异性体液和细胞免疫应答。结果:乙肝疫苗联合重组IL-12能明显增强小鼠T淋巴细胞的增殖活性、促进分泌细胞因子IFN-γ和IL-2并提高IgG2a抗体水平。结论:重组IL-12可显著增强乙肝疫苗诱导的细胞免疫应答,并使免疫应答转向Th1型。Objective To study the role played by recombinant IL - 12 in enhancing the intensity and quality of the immune response to hepatitis B vaccine in mice, and investigate the possibility of adding recombinant IL - 12 as adjuvants to hepatitis B therapeutic vaccine. Methods Recombitant IL - 12 was injected together with hepatitis B vaccine into mice and special anti - HBsAb in the mice and the cellular immune responses were examined. Results Recombinant IL - 12 can obviously enhance T lymphocyte multiplication activity, accelerate excretion of cytokines IFN-γ and IL- 2, and increase the IgG2a antibody in mice. Conclusion Recombinant IL- 12 can remarkably strengthen the cellular immune responses induced by the hepatitis B vaccine, and modulate the immune responses toward Thl.

关 键 词：乙型肝炎病毒 疫苗 免疫应答 白细胞介素-12 

分 类 号：R512.620.3[医药卫生—内科学]