固相萃取-HPLC法测定儿童血浆中咪达唑仑的浓度  

Determination of Midazolam in Children' s Plasma by Solid-phase Extraction-HPLC

作　　者：陆晓彤[1] 蒋樾廉 张顺国[1] 

机构地区：[1]上海交通大学医学院附属新华医院药剂科,上海市200092 [2]上海交通大学医学院附属上海儿童医学中心药剂科,上海市200147

出　　处：《中国药房》2007年第17期1326-1328,共3页China Pharmacy

摘　　要：目的:建立测定儿童血浆中咪达唑仑浓度的方法。方法:采用固相萃取-高效液相色谱法分别测定22例ICU机械通气患儿给药24h后血浆中咪达唑仑浓度。色谱柱为Zorbax Eclipse XDB C18,流动相为乙腈—7·56mmol·L-1硫酸铵溶液(梯度洗脱),柱温为25℃,流速为1·0mL·min-1,紫外检测波长为254nm。结果:咪达唑仑检测浓度在0·05～25·00mg·L-1范围内线性关系良好(r=0·9998);平均回收率为99·29%,日内和日间RSD均<4%;分析方法的最小定量限为0·01mg·L-1。结论:本方法灵敏、准确、简单、快速,可用于临床咪达唑仑血药浓度监测。OBJECTIVE： To develop a method for the determination of midazolam in child plasma.METHODS： Plasma level of midazolam in twenty two ICU children with mechanical ventilation was detected by solid - phase extraction - HPLC after treatment with midazolam for 24h.The separation was performed on Zorbax Eclipse XDB Cls column with acetonitrile - 7.56mmol · L^-1 ammonium sulfate（gradient elution） as mobile phase.The flow rate was 1.0mL · min^-1, the column temperature was 25℃ and the wavelength of UV detector was 254nm.RESULTS： The calibration curves for midazolam had a good linearity over the range of 0.05-25.00mg · L^-1（r = 0. 999 8） . The, ave, rage recovery for midazolam was 99.29% .Both the intra - day RSD and inter- day RSD were less than 4% .The limits of quantitation for midazolam was 0.01mg · L^-1.CONCLUSION：The method is sensitive,accurate,simple and rapid,and applicable for clinical blood drug level monitoring of midazolam.

关 键 词：咪达唑仑 固相萃取 高效液相色谱法 血药浓度 

分 类 号：R969.1[医药卫生—药理学]