脱氧鬼臼毒素对3龄菜青虫头部AChE、ATPase活性的影响  

Effects of deoxypodophyllotoxin on the activity of AchE and ATPase in the head of 3rd instar larvae of Pieris rapae L.

作　　者：张守刚[1] 刘艳青[1] 高蓉[1] 何斌[1] 赵鸿雁[1] 狄旭东[1] 程洁[1] 田瑄[2] 肖杭[1] 

机构地区：[1]南京医科大学应用毒理研究所神经毒理室,江苏南京210029 [2]兰州大学应用有机国家重点实验室,甘肃兰州730000

出　　处：《南京医科大学学报（自然科学版）》2007年第6期542-545,共4页Journal of Nanjing Medical University(Natural Sciences)

基　　金：国家自然科学基金资助项目(30371169;30571555;30671785);江苏省普通高校自然科学研究计划资助项目(02KJA330001)

摘　　要：目的:研究脱氧鬼臼毒素(DOP)对3龄菜青虫的毒杀作用及中枢神经系统AChE、ATPase活性的影响,探讨其杀虫机制。方法:采用小叶碟添加法,测定DOP对3龄菜青虫的毒杀活性,建立染毒模型,用试剂盒测定AChE和ATPase活性。结果:①DOP对3龄菜青虫的毒杀活性表现出浓度、时间依赖性,48h LC50=151.87mg/L>72h LC50=39.99mg/L>96h LC50=10.60mg/L。②DOP对AChE活性没有明显影响。③高浓度(125mg/L)组对ATPase活性持续抑制,中浓度(5、25mg/L)组在24h时激活,48h时恢复正常,72h时保持正常(5mg/L)或抑制作用(25mg/L)。结论:DOP对3龄菜青虫具有毒杀作用;3龄菜青虫中枢神经系统AChE不是DOP靶标,ATPase可能是DOP的重要靶标之一。Objective：To investigate toxicity of deoxypodophyllotoxin （DOP） against 3rd instar larvae of Pieris rapae L. and its effects on activities of AChE and ATPase, and to study its insecticidal mechanism. Methods：The toxicity of DOP was measured with leaf-dipping method. The activites of enzymes were determined with AChE and ATPase assay kit. Result： （1） DOP exhibited toxicity against 3rd instar larvae ofP. rapae L This toxicity is concentration-time-dependent with LC50=151.87 mg/L（at 48 h）〉LC50=39.99 mg/L （at 72 h）〉 LC50=10.60 mg/L （at 96 h）. （2） DOP did not affect the activity of ACHE. （3） DOP profoundly inhibited the activity of ATPase at high concentration（ 125 mg/L）. DOP at middle concentration（5,25 mg/L） showed activative effect at 24 h, then recovered at 48 h, and maintained normal （5 mg/L） or exhibited inhibitory effect （25 mg/L） at 72 h. Conclusion：DOP exhibited toxicity against 3rd instar larvae of P. rapae L AChE is not target of deoxypodophyllotoxin and ATPases may be one important target of deoxypodophyllotoxin.

关 键 词：脱氧鬼臼毒素 菜青虫 ACHE NA^+ -K^+ -ATPase Ca^2+ -Mg^2+ -ATPase 

分 类 号：R282.11[医药卫生—中药学] Q74[医药卫生—中医学]