Lims E:一个新的LIM同源域基因的克隆和初步分析  

Lims E:molecular cloning and characterization of a novel splice variant of the LIM domain-family gene

作　　者：程立波[1] 陈洁[2] 赵沐子[2] 刘庆淮[1] 李建民[2] 

机构地区：[1]南京医科大学第一附属医院眼科,江苏南京210008 [2]南京医科大学细胞生物学与医学遗传学系,江苏南京210029

出　　处：《南京医科大学学报（自然科学版）》2007年第6期550-554,共5页Journal of Nanjing Medical University(Natural Sciences)

基　　金：国家自然科学基金资助项目(30000088)

摘　　要：目的:克隆与分析小鼠不同剪切的Lims基因。方法:应用巢式RT-PCR,以小鼠cDNA为模板,扩增Lims基因不同剪切子,构入PinPointTM Xa-1T质粒,测序鉴定。结果:测序表明克隆了新的Lims基因变异剪切体Lims E,该变异剪切体编码区为1164bp,编码387个氨基酸。结论:比较基因组学分析显示,成功地克隆了一新的小鼠Lims基因剪切子Lims E,为进一步研究Lims基因在细胞发育中的功能打下了基础。Objective：To clone and analyze the different splicing variant of Lims gene in mouse. Methods：Splicing variants of Lims gene were amplified by RT-PCR from mouse cDNA and inserted into PinPointTM Xa-IT vector, two position clones selected by PCR were sequenced, Results：Lism E, a novel splicing variant with a 1164 bp open reading frame （ORF）, encoding a 387-amino acid （AA） protein was cloned. Conclusion：Comparative genome analysis displayed Lims E, a novel variant of Liras gene was confirmed in mouse and established the foundation for further to studying the function of Lims E in cell development.

关 键 词：Lims基因 基因表达 基因剪切 

分 类 号：R318.5[医药卫生—生物医学工程] Q74[医药卫生—基础医学]