GATA-4基因在大鼠胚胎心脏中的表达  

The expression and location of GATA-4 in the developing rat hearts

作　　者：孙凤荣[1] 龚杰[1] 钱玲梅[1] 孔祥清[1] 盛燕辉[1] 杨荣[1] 曹克将[1] 

机构地区：[1]南京医科大学第一附属医院心脏科,江苏南京210029

出　　处：《南京医科大学学报（自然科学版）》2007年第6期555-557,F0002,共4页Journal of Nanjing Medical University(Natural Sciences)

基　　金：国家自然科学基金资助项目(30470709);江苏省自然科学基金资助项目(BK2001043;BK2004156)

摘　　要：目的:检测GATA-4基因在大鼠胚胎心脏中的蛋白表达变化。方法:取大鼠胚胎12、15、19天心脏,应用Westernblot、免疫组化的方法进行半定量、定位分析。结果:免疫组化显示,GATA-4基因在房室肌、心内膜、心内膜垫、房室瓣及大动脉根部均有表达。Western blot显示,在胚胎12天心脏中,GATA-4基因已有较高表达;在胚胎15天表达明显上调,胚胎19天时表达又有所下降。结论:GATA-4随大鼠胚胎心脏的生长发育呈动态表达,GATA-4与心脏发育密切相关。Objective：To investigate the protein expression of GATA-4 in the developing rat hearts. Methods：Embryolc hearts of normal rats were obtained on the 12 th, 15 th and 19 th day during embryonic period. The expression level of GATA-4 protein was accessed by Western blot and Immunohistochemistry, and the results were analyzed by the statistical method of One-way ANOVA. Results：GATA-4 was expressed in atrial ventricular myocardium,endocardium, endocardial cushion and the root of main artery, The expression of GATA-4 in the heart was increasing obviously on 15 th day in embryonic period, but it was slightly decreasing on 19 th day. Conclusion：With the development of rat hearts, the protein expression of GATA-4 was dynamic. It was suggested that GATA-4 should play a crucial role in rat heart development.

关 键 词：GATA-4基因 大鼠 胚胎 心脏 

分 类 号：R322.11[医药卫生—人体解剖和组织胚胎学] Q74[医药卫生—基础医学]