普乐可复对肝癌细胞株HepG_2增殖的影响  

The effect of prograf on the hepatocellular carcinoma cell line HepG_2

作　　者：张长和[1] 李君[1] 葛文刚[1] 刘现忠[1] 仲跻巍[1] 李相成[1] 

机构地区：[1]南京医科大学第一附属医院肝脏外科,江苏南京210029

出　　处：《南京医科大学学报（自然科学版）》2007年第6期565-568,共4页Journal of Nanjing Medical University(Natural Sciences)

摘　　要：目的:研究普乐可复(即FK506)对肝癌肝移植术后体内可能残存肿瘤细胞的影响,从而对肝癌患者肝移植术后抗排异药物的选择起一定的指导作用。方法:在体外细胞培养条件下,通过MTT、RT-PCR、Western blot方法,研究普乐可复对肝癌细胞株HepG2增殖及其肿瘤相关细胞因子TGF-α和C-met的特异性表达的影响。结果:普乐可复组(5μg/L)对肝癌细胞株HepG2增殖及TGF-α及c-Met特异mRNA及蛋白的表达有抑制作用。与对照组相比,差异均有统计学意义(P<0.05)。结论:FK-506对肝癌细胞生长有一定的抑制作用。Objective：To investigate the effect of prograf on remnant hepatocellular carcinoma cells in the liver receptors after liver transplantation for liver cancer, and to instruct the anti-rejection drug selection. Methods：MTT assay, RT-PCR and Western blot were used to examine the expression of c-met and TGF-α in the hepatocellular carcinoma cell line HepG2 cultured in vitro. Results：The expression of c-met and TGF-αin prograf group（at concentration of 5 μg/L） was much lower than that in the control group（P 〈 0.05）. Conclusion： Prograf can inhibit the proliferation of hepatocellular carcinoma cell.

关 键 词：普乐可复 肝癌细胞 增殖 TGF-Α C-MET 

分 类 号：R657.3[医药卫生—外科学]