多重RT-PCR用于临床检测三种胃肠炎病毒的研究  

Studies on Simultaneous Detection of Three Gastroenteritis Virus in Clinical Samples by Muptiplex RT-PCR

作　　者：寇晓霞[1] 吴清平[1] 王大鹏[1] 郭伟鹏[1] 邓梅清[1] 

机构地区：[1]广东省微生物研究所广东省菌种保藏与应用重点实验室

出　　处：《微生物学通报》2007年第3期401-405,共5页Microbiology China

基　　金：广东省重大科技攻关项目(No.2002B3100103)

摘　　要：轮状病毒、诺瓦克病毒和星状病毒是引起病毒性胃肠炎的主要病原因子。研究采用JV12/JV13、P1/P2和Mon340/Mon348三对引物,建立了同时检测这3种病毒的多重RT-PCR技术,并应用于128份临床粪便样本的检测,检出轮状病毒62份(48.44%),诺瓦克病毒8份(6.25%),星状病毒11份(8.59%)。在灵敏度试验中,轮状病毒的检测灵敏度为5pg/mL、诺瓦克病毒和星状病毒的检测灵敏度均为50pg/mL。该研究所建立3种常见胃肠炎病毒的多重RT-PCR方法具有特异性强、灵敏度高的特点,可用于临床病原诊断和溯源。Rotavirus, norwalk virus and astrovirus are the major causes of viral gastroenteritis. Primers JV12/JV13, P1/P2 and Mon340/Mon348 were used to develop a multiplex RT-PCR system to simultaneously detect norwalk virus, rotavirus and astrovirus in clinical fecal samples. In a total of 128 samples, 62 was rotaviras, 8 was norwalk virus and ll was astrovirus, The detection limits was 5pg/mL for rotavirus, 50pg/mL for norwalk virus and astrovirus respectively, The multiplex RT-PCR system can be used to detect norwalk virus, rotavirus and astrovirus for routine monitoring and risk assessment in diseases outbreak with high specificity and sensitivity.

关 键 词：诺瓦克病毒 轮状病毒 星状病毒 多重RT-PCR 

分 类 号：R450[医药卫生—治疗学]