RAPD分子标记在酱油生产菌系统发育分析中的应用  

Application of RAPD Molecular Marker in the Phylogenetic Analysis of Soy Sauce Strains

作　　者：王斌[1] 潘力[1] 郭勇[1] 

机构地区：[1]华南理工大学生物科学与工程学院,广州510640

出　　处：《微生物学通报》2007年第3期508-511,共4页Microbiology China

基　　金：广东省自然科学基金项目(No.04020061);广东省科技攻关项目(No.2004B20201011)

摘　　要：以米曲霉沪酿3.042（AS3.951）、酱油曲霉AS3.495为参照,对从商品酱油曲精中分离到的8株酱油生产菌进行RAPD分析。实验筛选到6个扩增产物谱带多、特征好、覆盖面广的引物：Primer1、Primer5、Primer6、Primer7、Primer8、Primer9,重复实验证明其RAPD-PCR扩增图谱具有较好的稳定性,扩增产物谱带一般4～8条,各实验菌株主带1～4条,次带丰富。根据RAPD-PCR扩增图谱构建的系统进化树较好地吻合了传统的形态分类学,证实了RAPD分子标记在酱油生产菌系统发育分析中应用的可行性。RAPD analysis of eight soy sauce strains isolated from commercial soy sauce koji was done with random primers,using Aspergillus oryzae AS3.951 and Aspergillus sojae AS3.495 as controls. Six appropriate primers （ Primerl, Primer5, Primer6, Primer7, Primer8, Primer9） for RAPD-PCR were screened from nine random primers, and repetitive experiments demonstrated that their RAPD-PCR polymorphic patterns were stable. There were usually 4 - 8 bands in their RADP-PCR patterns, where the number of the main bands was 1 - 4 and the secondary bands were abundant. The phylogenetic tree of these strains was reconstructed according to their RAPD-PCR patterns, and it basically corresponded to traditional morphological taxology, demonstrating that the application of RAPD molecular marker in the phylogenetic analysis of soy sauce strains was feasible.

关 键 词：RAPD分子标记 酱油生产菌 系统发育分析 多态性 

分 类 号：Q931[生物学—微生物学] Q933