诱导分化剂诱导NB4来源的树突状细胞分化的研究  

Study on differentiation of dendritic cells (DC) from NB4 cell with inducing agent

作　　者：曹清[1] 陈同辛[1] 蒋黎明[1] 朱亚忠[1] 宋得莲[1] 

机构地区：[1]上海交通大学医学院附属新华医院上海儿童医学中心,上海200127

出　　处：《临床儿科杂志》2007年第6期488-491,共4页Journal of Clinical Pediatrics

摘　　要：目的研究全反式维A酸(ATRA)联合细胞因子体外诱导NB4细胞来源的树突状细胞(DC)的适宜方法。方法在NB4细胞中,分别加入ATRA、重组人粒细胞-巨噬细胞集落刺激因子(rhGM-CSF)+重组人白细胞介素-4(rhIL-4)、ATRA+rhGM-CSF+rhIL-4培养诱导10d。在培养第11天,取适量细胞进行免疫表型分析和形态学观察(所有实验均重复3次)。结果经ATRA+rhGM-CSF+rhIL-4培养10d,NB4-DC表面,HLA-ABC、CD1a、CD86表达明显增高,而CD33表达明显下降,与诱导前相比,差异有统计学意义(P<0.01),经光镜下观察细胞具有典型的树突状细胞形态学特征;NB4细胞经单独ATRA诱导后向正常髓系细胞分化和成熟。结论通过ATRA、rhGM-CSF及rhIL-4的培养体系可诱导NB4-DC。[临床儿科杂志,2007,25(6):488-491]Objectives To explore the feasibility of induction of dendritic cells from NB4 cells by combining all-trans retinoic acid （ATRA） and cytokines in vitro. Methods NB4 cells were co-cultured with ATRA, recombining human granulocyte macrophage colony-stimulating factor （rhGM-CSF）＋ recombining human interleukin-4 （rhIL-4）and ATRA＋rhGM-CSF＋rhIL-4. The morphology of the harvested cells was observed under light microscope, and the expression of phenotype was examined with flow cytometer on the 11 day of induction. All the processes were repeated for three times. Results The expressions of HLA-ABC, CD1a, CD86 were up-regulated, while the expression of CD33 was down-regulated significantly （ P 〈 0.01 ）. Typical dendritic cell morphology was found in expanded NB4 cell. However, NB4 cells being induced and differentiated into general granulocyte when they were induced with ATRA only. Conclusions The culture system with combination of ATRA, rhGM-CSF, and rhIL-4 can induce and expand NB4-dendritic cells effectively.

关 键 词：白血病 树突状细胞 分化 细胞因子 全反式维A酸 

分 类 号：R392[医药卫生—免疫学]